Glucocerebrosidase is a lysosomal enzyme that catalyzes the hydrolysis of glucosylceramide (GlcCer), a membrane glyco-sphingolipid, to ceramide and glucose. This assay detects GBA activity by using a fluorogenic substrate that reacts with cell…
Lange et al. proposed a model for mitochondrial prohibitin complex with unequal subunit stoichiometry. Data suggests an asymmetric structure, questioning the validity of the proposed model. Care is needed in symmetry imposition in cryo-ET.
