CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP

By Jiuchun Zhang and J. Wade Harper
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Output Details

Description
This protocol describes a method for knockin of a 3X flag tag onto the N-terminus of the EEA1 gene in human H9 ES cells using CAS9 and an ultramer oligonucleotide homologous recombination template
Identifier (DOI)
10.17504/protocols.io.kqdg3x99eg25/v1
Tags
  • CRISPR
  • Endosomes
  • Stem cells – embryonic
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

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