CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP

By Jiuchun Zhang and J. Wade Harper
View Published Protocol

Output Details

Description
This protocol describes a method for knockin of a 3X flag tag onto the N-terminus of the EEA1 gene in human H9 ES cells using CAS9 and an ultramer oligonucleotide homologous recombination template
Identifier (DOI)
10.17504/protocols.io.kqdg3x99eg25/v1
Tags
  • CRISPR
  • Endosomes
  • Stem cells – embryonic
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons

Protocol for depleting or tagging endogenous α-syn using virally-delivered CRISPR-Cas9 in hippocampal neurons

View Protocol

CRISPR tagging of the TMEM192 gene with 3xHA in H9 ES AAVS-NGN2 cells for Lyso-IP

Protocol for adding a 3X HA tag to TMEM192 gene in human cells using CAS9 and recombination template is described for H9 ES AAVS1-NGN2 cells.

View Protocol

CRISPR/Cas9 generation of knock-out iPSCs

This protocol describes RNP-based CRISPR/Cas9 gene-editing to generate knock-out iPSCs.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.