Datasets and Key Resources Table used in Bouabid, S. et al. (2025) “Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations””

### **ABSTRACT** Striatal acetylcholine (ACh) signaling is thought to counteract reinforcement signals, promoting extinction and behavioral flexibility. Changes in striatal ACh signals have been reported during learning, but how ACh signals for learning and extinction are spatially organized to enable region-specific plasticity is unclear. We used array photometry in mice to reveal a topography of opposing changes in ACh release across distinct striatal regions. Reward prediction error encoding was localized to specific phases of ACh dynamics in anterior dorsal striatum (aDS): positive and negative prediction errors were expressed in dips and elevations respectively. Silencing ACh release in aDS impaired extinction, suggesting a role for ACh elevations in down-regulating cue-reward associations. Dopamine release in aDS dipped for cues during extinction, inverse to ACh, while glutamate input onto cholinergic interneurons was unchanged. These findings pinpoint where and suggest an intrastriatal mechanism for how ACh dynamics shape region-specific plasticity to gate learning and promote extinction. ### **FILE DESCRIPTIONS** **FILE DESCRIPTIONS** This repository contains the following files: - _README_Source_Data (.txt) - File with detailed information on content within each individual .zip folder that relates to a figure. - Key Resources Table (.xlsx) - Table containing details on key resources (mouse lines, virus strains, software), and the persistent identifiers for protocols and MATLAB custom-written code used and generated in this study. - Fig1 to 8 (.zip) - Individual folders contaning raw preprocessed and processed datasets for each Main Figures 1 to 8. - Supp Fig1 to 7 (.zip) - Individual folders contaning raw preprocessed and processed datasets for each Supplementary Figures 1 to 7.