Fabrication of fluid-walled dumbbells and generation of the human corticostriatal pathway

By Federico Nebuloni, Quyen Do and Richard Wade-Martins, PhD
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Output Details

Description
This protocol described the generation of fluid-walled dumbbells for culturing of induced pluripotent stem cell-derived neurons with directional connectivity so as to recapitulate the human corticostriatal connectivity. The fluid-walled microfluidic technology was first reported by Walsh et al., 2017 and subsequently shown to be adaptable for cell culture.
Identifier (DOI)
10.17504/protocols.io.kqdg39zb1g25/v1
Tags
  • 2D cultures
  • Axons
  • Cell culture
  • hiPSCs (Human induced pluripotent stem cells)
  • Microfluidic devices
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

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