This study employs a novel drug targeting technology DART(drug acutely restricted by tethering), to selectively block GABAA receptors on VTADA neurons as mice engage in Pavlovian learning.
Protocol labels ChAT for cholinergic interneurons and TH for dopaminergic neurons in mouse brain slices. Immunofluorescence used post ex vivo voltammetry recordings on 300-µm thick slices.
This protocol is to assess changes in extracellular dopamine concentration following electrical or optogenetic stimulations in ex vivo mouse brain slices.
