Figure S2. pS72 Rab7 formation following depolarization, starvation, and iron chelation [FINAL]

Figure S2. pS72 Rab7 formation following depolarization, starvation, and iron chelation. (A) Parkin-expressing HeLa cells were treated for 1 H with either growth medium, growth medium supplemented with oligomycin/antimycin, Earle’s Balanced Salt Solution, or growth medium supplemented with deferiprone, fixed, and then labelled using an antibody specific for pS72 Rab7. (B) Quantification of pRab7 signal. Images of cells were backgrounded subtracted, cells were masked, and total fluorescence intensity was measured on a per cell basis. At least 15 cells were measured in this fashion, and averaged, constituting a single biological replicate (outlined, colored circles). This was repeated for a total of 3 independent replicates for each condition. Horizontal black bars indicate averages of the 3 replicates. Colors indicate replicates performed on the same day. P-value was determined using a paired, one-tailed T-test.