Genetic Modification of Human Induced Pluripotent Stem Cells (hiPSCs) by Lentiviral Transduction Protocol

By Roshni Jaffery, BSc, Ningbo Zheng, PhD, Jiakai Hou, PhD, Ashley Guerrero, Si Chen, Chunyu Xu, PhD, Nicholas A. Egan, Ritu Bohat and Weiyi Peng, PhD
View Published Protocol

Output Details

Description
This is the protocol for the genetic modification of human induced pluripotent stem cells (hiPSCs) using lentiviral transduction method.
Tags
  • Genetic models
  • Genetic targets
  • hiPSCs (Human induced pluripotent stem cells)
Team
Team Chen
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

  • Roshni Jaffery, BSc

    Key Personnel: Team Chen, 

    University of Houston

  • User avatar fallback logo

    Ningbo Zheng, PhD

    Key Personnel: Team Chen, 

    University of Houston

  • Jiakai Hou, PhD

    Key Personnel: Team Chen, 

    University of Houston

  • User avatar fallback logo

    Ashley Guerrero

  • User avatar fallback logo

    Si Chen

  • User avatar fallback logo

    Chunyu Xu, PhD

    Key Personnel: Team Chen, 

    University of Houston

  • User avatar fallback logo

    Nicholas A. Egan

  • User avatar fallback logo

    Ritu Bohat

  • Weiyi Peng, PhD

    Co-PI (Core Leadership): Team Chen, 

    University of Houston

Related Research

Tamoxifen Administration in Transgenic Mice-Ding Lab

This protocol describes the procedure for inducing CreER-mediated recombination at loxP sites in transgenic mice through the administration of tamoxifen or 4-hydroxytamoxifen (4-OHT).

View Protocol

Transcriptional programs mediating neuronal toxicity and altered glial-neuronal signaling in a Drosophila knock-in tauopathy model

Mutations in gene encoding tau cause autosomal dominant forms of frontotemporal dementia. This article presents a genetic model of tauopathy, which recapitulates the genetic context and phenotypic features of the disease.

View Article

Passaging and Seeding Mouse Embryonic Fibroblasts (MEFs) for Experiments

Protocol for passaging and seeding mouse embryonic fibroblasts (MEFs) outlined.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.