GUV assay

By Chunmei Chang
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Output Details

Description
This protocol describes the preparation of GUVs for the reconstitution of LC3 lipidation reaction through the steps of PI3P production by PI3KC3-C1, WIPI2 recruitment and LC3 lipidation with PE.                             
Identifier (DOI)
10.17504/protocols.io.bxm2pk8e
Tags
  • Biochemistry
  • LC3 lipidation
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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    Chunmei Chang

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