HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3

ID   HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 AC   CVCL_D4BX SY   Syn10 Galectin-3 FRET             CC   Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). CC   Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission). CC   Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). CC   Transfected with: HGNC; 6563; LGALS3. CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). CC   Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3). CC   Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Ser65Gly, p.Gln69Ala, p.Phe99Ser, p.Asn105Thr, p.Tyr145Phe, p.Asn149Tyr, p.Met153Thr, p.Gly160Cys, p.Val163Ala, p.Ile171Val, p.Thr203His, p.Ala206Lys = mClover3). CC   Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo. CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5. CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA]. CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa P. CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. OX   NCBI_TaxID=9606; ! Homo sapiens (Human) hi   CVCL_D4BW ! HEK293T TetOff-SNCA-A53T SX   Female AG   Fetus CA   Transformed cell line Parental cell line: HEK293T (RRID:CVCL_0063)