HyDrop-RNA v1.0

By Florian De Rop, MSc, Stein Aerts, PhD and Suresh Poovathingal
View Published Protocol

Output Details

Description
Step-by-step protocol for performing HyDrop-RNA. The duration of each step assumes an experienced protocol user. For a first-time user, we recommend doubling the expected time for each step. Visit [https://hydrop.aertslab.org/ ](https://hydrop.aertslab.org/)for more info
Identifier (DOI)
10.17504/protocols.io.dm6gpwqjjlzp/v5
Tags
  • RNA
  • scRNAseq (Single-cell RNA-seq)
  • Single-cell
Team
Team Voet
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

Related Research

Hydrop enables droplet-based single-cell ATAC-seq and single-cell RNA-seq using dissolvable hydrogel beads

HyDrop protocol enables single-cell sequencing for RNA and chromatin accessibility. Generated over 7996 single-cell profiles for ATAC-seq and 9508 for RNA-seq in mouse cortex. Protocol validated on low-input samples from Drosophila brain.

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HyDrop-ATAC v1.0

Step-by-step protocol for execution of HyDrop-ATAC.

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HyDrop Bead Generation & PCR Barcoding v1.0

Protocol for producing dissolvable barcoded hydrogel beads used in HyDrop experiments.

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