Poly-ornithine/laminin substrate for neural cell culture V.2

By Regine Tipon, MSc
View Published Protocol

Output Details

Description
This protocol is used to create adhesive and bioactive substrate for neural cell types, low to high density neurons, astrocytes, or organoids. It is based on standard methods but includes several optimizations, use case recommendations, and alternatives, and advice.
Identifier (DOI)
10.17504/protocols.io.dm6gpz2mplzp/v2
Tags
  • 2D cultures
  • 3D cultures
Team
Team Studer
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Regine Tipon, MSc

    Key Personnel: Team Studer, 

    New York Stem Cell Foundation

Related Research

Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”

The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.

View Dataset

Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation

The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.

View Article

STC-1 cell culture

Cell culture of STC-1 mouse enteroendocrine cells.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
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