Proteomic data reported in doi.org/10.1073/pnas.2219953120 (Golgi-IP, a tool for multimodal analysis of Golgi molecular content)

By Raja Sekhar Nirujogi, PhD and Rotimi Fasimoye, PhD
View Published Dataset

Output Details

Description
Proteomic data associated with doi.org/10.1073/pnas.2219953120 deposited in ProteomeXchange PRIDE repository with PID: PXD038046.
Tags
  • Golgi apparatus
  • Mass spectrometry
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Raja Sekhar Nirujogi, PhD

    Key Personnel: Team Alessi, 

    University of Dundee

  • Rotimi Fasimoye, PhD

    Key Personnel: Team Alessi, 

    University of Dundee

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Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content

The authors develop a method for the rapid capture of intact Golgi via Golgi immunoprecipitation. Using high-resolution mass spectrometry, the approach allows the unbiased characterization of the Golgi proteome, metabolome, and lipidome.

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HEK293 TMEM115-3xHA GolgiTAG (CVCL_C8A6)

Cell line generated from HEK293 cells (Cat# CRL-1573, RRID:CVCL_0045) virally transfected with a construct expressing TMEM115-3xHA (GolgiTAG)

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Staining of cells with GolgiTracker for Golgi flow cytometry analysis

Method that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis.

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