Sample Collection and Measurement of Serum Neurofilament Light (NfL)

By Huw Morris, Donald Grosset, Amanda Heslegrave and Henrik Zetterberg
View Published Protocol

Output Details

Description

This protocol details the steps for the preparation of serum from human blood samples and the measurement of NfL concentration using the NF-Light Advantage kit on the HD-X Analyzer (Quanterix, Billerica, MA).

Identifier (DOI)
10.17504/protocols.io.bzbep2je
Tags
  • Blood
  • Ex Vivo
  • Human
  • NfL (Neurofilament light)
  • Parkinson's disease
  • Protein
Team
Team Hardy
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Huw Morris

    Collaborating PI: Team Hardy, 

    University College London

  • User avatar fallback logo

    Donald Grosset

  • User avatar fallback logo

    Amanda Heslegrave

  • User avatar fallback logo

    Henrik Zetterberg

Related Research

Proband-NFL Analysis

Code used for the manuscript "Combining biomarkers for prognostic modelling of Parkinson’s disease" by Niro Viijaratnam and colleagues.

View Code

Inflamed Microglia like Macrophages in the Central Nervous System of Prodromal Parkinson′s Disease

We investigated the role of inflammation in the pathogenesis of prodromal PD performing single-cell RNAseq analysis of CSF and blood from 111 individuals.

View Article

SNP Genotyping and ApoE Genotyping

Protocol outlines DNA extraction from blood, quality control, SNP, and APOE genotyping. Adapted from PRoBaND SNP Genotyping and ApoE Genotyping Protocol by Malek et al. for Parkinson's Disease study.

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.