Systemic AAV administration through retro-orbital injection

Output Details

Adeno-associated viruses (AAVs) are popular tools for gene delivery to animal models. Natural and engineered AAVs can even achieve widespread transduction of target organs through systemic delivery. Fast (<3 minute per mouse) and reproducible transduction through systemic AAV delivery can be achieved with retro-orbital injection. This protocol describes how to systemically deliver AAVs via the retro-orbital sinus.
Tags
  • AAV
  • AAV (Adenoassociated Virus)
  • In Vivo
  • Mouse

Meet the Authors

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    Gerard Coughlin, BSc

    Key Personnel: Team Gradinaru

    California Institute of Technology