Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD
By onThe regulatory mechanisms that prevent autophagy pathways from overreacting are largely elusive. The authors shed light on a cellular strategy to prevent pathway hyperactivity while still ensuring efficient progression.
Immunohistochemistry for brain sections
By onProtocol for processing 30um mouse brain sections for immunolabeling.
Data sets related to “Kufor-Rakeb Syndrome-Associated Psychosis: A Novel Loss-of-Function ATP13A2 Variant and Response to Antipsychotic Therapy”
By onData sets related to "Kufor-Rakeb Syndrome-Associated Psychosis: A Novel Loss-of-Function ATP13A2 Variant and Response to Antipsychotic Therapy."
An ImageJ macro for the quantification of ciliary accessible cholesterol
By onALOD4 probe visualizes cholesterol pool; algorithm segments cilia using SSTR3 marker. Masks determine ALOD4-bound cholesterol intensity in ciliary membrane from .tif-files. SSTR3-positive cilia identified, intensities measured in green channel.
Primary data associated with the manuscript “Restoration of striatal neuroprotective pathways by kinase inhibitor treatment of Parkinson’s linked-LRRK2 mutant mice” (doi: 10.1101/2024.07.31.606089)
By onPrimary data associated with the manuscript "Restoration of striatal neuroprotective pathways by kinase inhibitor treatment of Parkinson’s linked-LRRK2 mutant mice" (doi: 10.1101/2024.07.31.606089).
Immunostaining- Fluorescent
By onStaining of mouse brain sections with immunofluorescent visualization.
Imaging- Bright field
By onImaging protocol for Hamamatsu NanoZoomer slide scanner: Mount sections on slides, stain with markers, and coverslip. Analysis with Carl Zeiss LSM 880 confocal microscope.
Imaging- Confocal
By onProtocol for imaging using confocal microscope. Sections for analysis should be mounted on slides, stained for appropriate markers, and coverslipped. This protocol is using a Carl Zeiss LSM 880 confocal microcope.
Protocol for quantification of neurons expressing alpha-synuclein in the non-human primate brain
By onThe intraputaminal delivery of AAV9-SynA53T resulted in a widespread synucleinopathy throughout the cerebral cortex and substantia nigra in the non-human primate brain.
Raw tabular data of confocal microscopic images for “Characterizing enteric neurons in dopamine transporter (DAT)-Cre reporter mice reveals dopaminergic subtypes with dual-transmitter content”
By onUsing immunofluorescence we uncovered a subpopulation of tdTomato-immunoreactive neurons in the ENS exhibit dual-transmitter content (DAT/ChAT, DAT,nNOS)
Hematoxylin and Eosin Stain – FOR PARAFFIN-EMBEDDED TISSUE SECTIONS
By onProtocol for Hematoxylin and Eosin Stain on paraffin-embedded tissue sections is described.
DAB Staining of Fixed Mouse Brain Tissue Sections
By onThis protocol describes steps for immunohistochemical 1 3,3’-Diaminobenzidine (DAB) staining of free floating fixed mouse brain tissue sections.
Immunofluorescence Staining of Fixed Mouse Brain Tissue Sections
By onThis protocol describes steps for immunofluorescent staining of free floating fixed mouse brain tissue sections.
Optical Density Analysis for DAB Staining of Mouse Brain Sections
By onThis protocol describes steps for optical density analysis of immunohistochemical 3,3′-Diaminobenzidine (DAB) staining of mouse brain sections using ImageJ.
Data sets related to “Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits””
By onData sets related to "Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits"
Pixel-dependent offset, gain, variance and readnoise maps for CS505MUP polarisation camera
By onFour tif files are the pixel-dependent camera offset (in ADU counts), gain (in ADU counts/photoelectron), variance (in ADU counts^2) and readout noise (in photoelectrons) for the full sensor of a polarization camera (CS505MUP, Thorlabs)
Polarisation camera dSTORM datasets of actin in fixed HeLa cells labeled with phalloidin-Alexa Fluor 647
By onPolarisation camera dSTORM dataset of the actin of fixed HeLa cells labeled with phalloidin-Alexa Fluor 647.
Polarisation camera dSTORM datasets of actin in fixed HeLa cells labeled with phalloidin-Alexa Fluor 488
By onPolarisation camera dSTORM dataset of the actin of fixed HeLa cells labeled with phalloidin-Alexa Fluor 488.
Polarisation camera PAINT movie of single Nile red dyes binding to lipid bilayer-coated silica microspheres
By onThis PAINT dataset is a movie of the fluorescence of single Nile red molecules reversibly binding to a lipid bilayer (DPPC + 40% cholesterol) on a 5 μm diameter silica microsphere (44054-5ML-F, Sigma-Aldrich).
