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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Code
FIJI Syn_Bot Macro
At a neuronal synapse, presynaptic and postsynaptic terminals are ~50nm apart, and thus immunohistochemical staining of a protein from each compartment will give a colocalized signal due to the resolution limits of traditional fluorescence microscopy. This macro counts the number of colocalized synaptic puncta in fluorescence microscopy images.
Teams
Code
Installing FIJI and SynBot (Windows version)
Video instructions for installing FIJI and SynBot and a simple SynBot run for Windows operating system.
Teams
Code
ilastik install and run for Syn Bot (Mac version)
Video instructions for installing ilastik, training an ilastik project, and using the ilastik project for simple SynBot run for Mac operating system.
Teams
Code
ilastik install and run for Syn Bot (Windows version)
Video instructions for installing ilastik, training an ilastik project, and using the ilastik project for simple SynBot run for Windows operating system.
Teams
Code
Synapse quantification with Puncta Analyzer
Protocol for installing Puncta Analyzer and using it to analyze synapse images.
Teams
Code
R scripts for the statistical analysis and plotting in the paper GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes
R scripts for the statistical analysis and plotting in the paper GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes.
Teams