Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input
The authors explored the cellular and synaptic mechanisms of levodopa-induced dyskinesia (LID), a complication of Parkinson’s disease therapy characterized by involuntary movements. Findings suggest how the intrinsic and synaptic properties of heterogeneous dMSN subpopulations integrate to support action selection.
Primary data for article, “Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input” (DOI: 10.1016/j.celrep.2024.114483)
Primary data for the published manuscript, “Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input” (Ryan et al. Cell Reports 43:8, 114483, 2024.).
Immunostaining mouse brain tissue or neuronal cultures
This protocol describes immunohistochemical and immunocytochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.
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Therapeutic deep brain stimulation disrupts movement-related subthalamic nucleus activity in Parkinsonian mice
Publication: Subthalamic nucleus deep brain stimulation relieves many motor symptoms of Parkinson’s disease, but its underlying therapeutic mechanisms remain unclear. Here, authors used electrical artifact-free GCaMP fiber photometry to investigate activity in basal ganglia nuclei during STN DBS in parkinsonian mice to get at that question. View original preprint.
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Immunohistochemistry
This protocol describes immunohistochemical staining of fixed brain sections.
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In Vivo Electrophysiology (Mouse)
This protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
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Optogenetic Manipulation (Mouse)
This protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
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Mouse Stereotaxic Surgery
This protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
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Behavioral Testing – Open Field and Dyskinesia Scoring
This protocol describes behavioral testing to assess motor deficits in mice. It includes open field locomotor testing and scoring of levodopa-induced dyskinesia.
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Live imaging of primary mouse neuron cultures
This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging technique was used in Jain et al., 2023 to compare vesicular release in neurons between various transgenic knockout mouse lines.
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Ex Vivo Electrophysiology
This protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
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Mouse behavior – Open Field and T-Maze
This protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
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Mouse brain tissue collection and analysis
This protocol describes the dissection and collection of coronal sections of the striatum and midbrain from a mouse brain.
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Photometry analysis code
This Matlab script extracts GCaMP fiber photometry data and computes the number and amplitude of transients over time.
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