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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Mouse Stereotaxic Surgery
This protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
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Article
Dopamine across timescales and cell types: Relevance for phenotypes in Parkinson’s disease progression
This review covers recent conceptual advances in our basic understanding of the dopamine system – including our rapidly advancing knowledge of dopamine neuron heterogeneity – with special attention to their importance for understanding PD.
Teams
Protocol
Deep Brain Stimulation (DBS) Implant
This protocol describes the steps for making implants used for deep brain stimulation (DBS) in rodents.
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Protocol
Ex Vivo Electrophysiology
This protocol describes preparation of brain slices, setup of electrophysiology rig, and solutions for collecting whole cell and cell attached recordings.
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Protocol
Fiber Photometry (Mouse)
This protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.
Teams
Article
Therapeutic deep brain stimulation disrupts movement-related subthalamic nucleus activity in Parkinsonian mice
Publication: Subthalamic nucleus deep brain stimulation relieves many motor symptoms of Parkinson’s disease, but its underlying therapeutic mechanisms remain unclear. Here, authors used electrical artifact-free GCaMP fiber photometry to investigate activity in basal ganglia nuclei during STN DBS in parkinsonian mice to get at that question. View original preprint.
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Protocol
Immunohistochemistry
This protocol describes immunohistochemical staining of fixed brain sections.
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Protocol
In Vivo Electrophysiology (Mouse)
This protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
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Protocol
Optogenetic Manipulation (Mouse)
This protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
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Protocol
Live imaging of primary mouse neuron cultures
This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging technique was used in Jain et al., 2023 to compare vesicular release in neurons between various transgenic knockout mouse lines.
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Protocol
Fast Scan Cyclic Voltammetry (FSCV) in mouse brain slices
This protocol describes the method of Fast Scan Cyclic Voltammetry (FSCV) for the application of measuring dopamine transients in mouse brain slices. Within the protocol are sections describing the manufacture of microelectrodes, preparation of acute brain slices, fiber calibration, and measuring stimulated dopamine release.
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Article
M4-mediated cholinergic transmission is reduced in Parkinsonian mice and its restoration alleviates motor deficits and levodopa-induced dyskinesia
Preprint: Despite M4-receptors being thought to mediate anti-kinetic effects, restoring M4-receptor function partially rescued Parkinsonian balance and coordination deficits and limited the development of levodopa-induced dyskinetic behaviors, indicating that decreased M4-function contributed to circuit and motor dysfunctions in response to DA loss.
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Protocol
Immunostaining mouse brain tissue or neuronal cultures
This protocol describes immunohistochemical and immunocytochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.
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Article
Alterations in neurotransmitter co-release in Parkinson’s disease
This review summarizes previous work characterizing neurotransmitter co-release from dopamine neurons, work examining potential changes in co-release dynamics that result in animal models of Parkinson’s disease, and future opportunities for determining how dysfunction in co-release may contribute to circuit dysfunction in Parkinson’s disease.
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