Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
AAV injection in the nodose ganglia of mouse
The study of these ganglia is challenging in small animals due to size and location. Hence, in this protocol, the team describes a practical surgical approach to the vagal trunk, and the JNG complex in mice.
Teams
Source data for “α-Synuclein pathology disrupts mitochondrial function in dopaminergic and cholinergic neurons at-risk in Parkinson’s disease”
Images and raw data for the figures in the paper, “α-Synuclein pathology disrupts mitochondrial function in dopaminergic and cholinergic neurons at-risk in Parkinson’s disease.”
Operant behavior
This protocol is for training mice operant conditioning to assess cognitive performance using the resources described in Matikainen-Ankney BA et al. 2021.
Teams
Bioluminescence imaging
This protocol describes a bioluminescence stepwise imaging protocol in mice with AAV-luciferase expression in the vagal afferents using the Ivis Lumina series III imaging system.
Teams
Gut-initiated alpha synuclein fibrils drive Parkinson’s disease phenotypes: Temporal mapping of non-motor symptoms and REM sleep behavior disorder
This study assesses the complex connections between α-synucleinopathies, gut-brain connectivity, and the emergence of non-motor phenotypes.
Teams
Immunohistochemistry for brain sections
Protocol for processing 30um mouse brain sections for immunolabeling.
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Cell counting
Protocol for counting cells labeled with immunofluorescent markers in mouse brain sections. Sections should be stained, mounted, and imaged with high resolution (2048 x 2048 scanning).
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Imaging – Bright field
Protocol for imaging using Hamamatsu NanoZoomer slide scanner. Sections for analysis should be mounted on slides, stained for appropriate markers, and coverslipped. This protocol uses a Carl Zeiss LSM 880 confocal microscope.
Teams
Imaging – Confocal
Protocol for imaging using confocal microscope. Sections for analysis should be mounted on slides, stained for appropriate markers, and coverslipped. This protocol uses a Carl Zeiss LSM 880 confocal microscope.
Teams
Immunostaining – Fluorescent
Staining of mouse brain sections with immunofluorescent visualization.
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Gut-PFF surgery
Method for injection of alpha-synuclein preformed fibrils in the gut in a mice.
Teams
Production of α-synuclein preformed fibrils (PFF)
This protocol outlines the procedure to produce preformed fibrils (PFF).
It has been adapted from Volpicelli-Daley et al., 2014.
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