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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Lab Resource
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
Plasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter.
Generated by Vectorbuilder in the pLV[Exp] backbone.
Teams
Themes
Lab Resource
Lentivirus plasmid: pLV[Exp]-U6>KANSL1_P1_Seq1-hPGK>mApple
Plasmid vector encoding a sgRNA sequence which targets the KANSL1 promoter from CRISPRi, under a U6 promoter and a mApple fluorescent reporter.
Generated by Vectorbuilder in the pLV[Exp] backbone.
Teams
Themes
Lab Resource
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
Plasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter.
Generated by Vectorbuilder in the pLV[Exp] backbone.
Teams
Themes
Lab Resource
Lentivirus plasmids for sgRNA: pLV[Exp]-U6>NT-Seq1-hPGK>mApple
Plasmid vector encoding a non-targeting sgRNA sequence under a U6 promoter and a mApple fluorescent reporter.
Generated by Vectorbuilder in the pLV[Exp] backbone
Teams
Themes
Lab Resource
Plasmid: pLV[Exp]-EF1A>V5/hKAT8[NM_032188.3]:IRES:Puro
Human KAT8 gene with N-terminal V5 tag cloned into the pLV[Exp] plasmid backbone under a EF1a promoter and including IRES puromycin selection cassette by Vectorbuilder.
Teams
Themes
Lab Resource
Plasmid: V5-KANSL1 pLVX-EF1α-IRES-Puro
Human KANSL1 cDNA with N-terminal V5 tag cloned into the pLVX-EF1α-IRES-Puro plasmid (EcoRI and SpeI restriction sites) from Clontech, Takara Bio.
Teams
Themes
Lab Resource
Lentivirus plasmid: pLV[Exp]-U6>PINK1_P1_Seq3-hPGK>mApple
Plasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter.
Generated by Vectorbuilder in the pLV[Exp] backbone.
Teams
Themes