Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Protocol

Single cell/nuclei RNAseq analysis

This protocol describes the process for the single cell/nuclei RNA sequencing data of the manuscript, “L1retrotransposons drive human neuronal transcriptome complexity and functional diversification,” from fetal forebrain and adult prefrontal cortex tissue.

Protocol

Isolation of NeuN+ cells from brain tissue (for CUT and RUN)

This protocol describes the steps to isolate NeuN+ cells from brain tissue in preparation for CUT and RUN.

Protocol

Iso-Seq mapping to L1HS/PA2 consensus sequence

The protocol describes the steps to map HiFi reads to a consensus sequence and retrieve density plots.

Protocol

Bulk RNA sequencing analysis

This protocol describes the steps for the bioinformatical analysis of bulk RNA sequencing with a focus on evolutionary young L1s.