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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Dataset
Primary data associated with the manuscript 10.1126/science.adi9926 (“Rab29-dependent asymmetrical activation of leucine-rich repeat kinase 2”)
Primary data associated with the manuscript “Rab29-dependent asymmetrical activation of leucine-rich repeat kinase 2” (Hanwen Zhu, Francesca Tonelli, Martin Turk, Alan Prescott, Dario R. Alessi, Ji Sun). These include immunoblotting data and their quantitation, representative confocal microscopy images, and quantitation and statistical analysis of confocal microscopy images.
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Protocol
Standard operating procedure for the isolation of genetically engineered hPSCs clones in a high-throughput way
This collection describes a standard procedure for isolating single human pluripotent stem cell (hPSC) clones in a high-throughput way. This collection follows nucleofection of hPSCs as described in detail in the collection “Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs.” dx.doi.org/10.17504/protocols.io.b4qnqvve
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Protocol
Amplifying the target genomic region by PCR
This protocol describes a procedure for amplifying targeted genomic regions using a PCR reaction.
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Themes
Tool
pBPK1520-SNCA-A30P-ng
It can be used to introduce SNCA-A30P mutation using prime editing, PE3 approach.
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Themes
Tool
pBPK1520-SNCA-A53T-ng
It can be used to introduce SNCA-A53T mutation using prime editing, PE3 approach.
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Dataset
Bulk NGS/allele quantification – Highly efficient generation of isogenic pluripotent stem cell models using prime editing
This file contains sequencing results for amplicons covering specific regions of interest related to prime editing of hPSCs.
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Lab Resource
BPK1520-LRRK2-G2019S-ng
This resource can be used to introduce LRRK2-G2019S mutation using prime editing, in PE3 approach.
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Lab Resource
pU6-pegRNA-LRRK2-G2019S-3d
This resource can be used to introduce LRRK2-G2019S mutation using prime editing.
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Lab Resource
pU6-pegRNA-LRRK2-G2019S-3c
This resource can be used to introduce LRRK2-G2019S mutation using prime editing.
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Lab Resource
pU6-pegRNA-LRRK2-G2019S-3b
This resource can be used to introduce LRRK2-G2019S mutation using prime editing.
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Lab Resource
pU6-pegRNA-LRRK2-G2019S-3a
This resource can be used to introduce LRRK2-G2019S mutation using prime editing.
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Lab Resource
Px330-EGFP-LRRK2-CRISPR/Cas9
This resource can be used to introduce LRRK2-G2019S mutation using CRISPR/Cas9 based genome editing.
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Lab Resource
pBPK1520-HEK3-CTTins-ng
This resource can be used to introduce HEK3 CTT insertion using prime editing, PE3 approach. It serves as a good positive control for prime editing.
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Protocol
AAVS1 Knock-in
This protocol describes the standard procedure to knock-in constructs to the AAVS1 safe harbor locus in hPSCs.
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