Structural conversion of α-synuclein at the mitochondria induces neuronal toxicity; Data sets
By onStudy includes data sets on α-synuclein structural changes in mitochondria causing neuronal toxicity. Data covers imaging, LCMS, sequencing, assays, fluorescence, redox, ATP, cell death, and more.
Raw immunocytochemistry images and quantification of CASR expression from Kilfeather & Khoo et al. (2024) “Single-cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease”
By onRaw immunocytochemistry images and quantification of CASR expression in mouse brain slices.
Stereo-seq raw data of the mouse ventral midbrain with age and alpha-synuclein overexpression
By onStereo-seq raw data from 3- and 18-month old mice, with and without human alpha-synuclein overexpression.
Data for outlier-waveform-detection of internal globus pallidus (GPi) activity
By onThis repository contains data based on neuronal recordings from two monkeys (G and I, in the pre- and post-MPTP states) that serve as input to the code provided at .
Calbindin KD Mouse – Experimental Cross
By onTransgenic mouse model with decreased calb1 expression in DAT-expressing cells.
Ultra-high field, multi-echo MRI acquisition for resting-state functional connectivity
By onMRI acquisition sequence at USC's Center for Image Acquisition examines motor circuits in Parkinson’s Disease using a Siemens 7T Terra scanner. Scans include anatomical T1 MPRAGE, spin-echo EPI, and multi-echo EPI sequences.
Outlier waveform detection for internal globus pallidus (GPi) activity
By onMatlab code identifies single units with spike waveforms that take on outlier feature values in the internal segment of the globus pallidus (GPi) units recorded from two nonhuman primates (in healthy and parkinsonian states).
Seeded amplification assay (SAA) from neuronal cell lysates
By onSeeded amplification assay (SAA) from neuronal cell lysates
Induced Pluripotent Stem Cells: A Tool for Modelling Parkinson’s Disease
By onPD involves loss of dopaminergic neurons in the substantia nigra. Patient-derived iPSC models aid in understanding the disease and identifying potential treatments. More research is needed on age-related aspects and gene-environment interactions.
Code for: Dissociation of putative open loop circuit from ventral putamen to motor cortical areas in humans I: high-resolution connectomics
By onCode for pre-processing fMRI data and region on interest masks associated with Rixor et al. 2024.
Immunofluorescence and microscopy of iPSC-derived cortical neurons
By onImmunofluorescence and microscopy of iPSC-derived cortical neurons protocol for the preservation of lipid-rich inclusions.
Induced Cortical Neuron Differentiation – Part 3 – Day 7 Dissociation
By onProtocol for replating neurons after seven days of cortical transdifferentiation and prepare cells for longer-term culture.
Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
By onDetection of minute amounts of misfolded α-synuclein aggregates in neuronal cell lysates.
Western blot of amplified fibrils after Proteinase K digestion
By onProtocol for western blot of amplified fibrils of a-synuclein after proteinase K digestion.
Induced Cortical Neuron Differentiation – Part 2 – Day 0 Dissociation
By onProtocol to initiate differentiation of human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puroSNAP PiggyBac construct, with a blasticidin-resistance backbone.
Induced Cortical Neuron Differentiation – Part 1 – Overview
By onProtocol to differentiate human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puro-SNAP PiggyBac construct, with a blasticidin-resistance backbone.
Seahorse XF Cell Mito Stress Test
By onSeahorse XF Cell Mito Stress Test for assessment of oxygen consumption rate in iPSC-derived neurons as a measure of mitochondrial respiration.
Quantitative PCR for STMN2 expression in pi-N neurons
By onQuantitative PCR for STMN2 expression in pi-NSNCA-STMN2 neurons.
PFF seeding in U2OS cells
By onThis protocol describes the seeding of intracellular inclusions in U2OS cells harboring overexpressing a-synuclein via exogenous addition of short-length preformed a-synuclein fibrils.
