Zhai et al. 2025 Raw data
By onData includes immunohistochemistry images, two-photon images of patched neurons, whole-cell recordings for somatic excitability and Sr2+-oEPSC, spine density imaging, ACh sensor imaging, and dendritic excitability linescan data.
Assay for lysosomal activity using Magic Red
By onAssay for lysosomal Cathepsin B activity in neurons using Magic Red
Immunoprecipitation from primary neurons
By onProtocol for immunoprecipitating proteins from neuronal lysates
Mitophagy detection assay
By onDetection of mitophagy flux in neurons using Dojindo Mtphagy dye and Lysotracker Green
A lipid transfer-dependent feedback loop activates ATG9A compartments in autophagy initiation
By onPhagophore formation requires ATG9, ATG2 and PI3KC3-C1. Authors show that ATG2 is crucial for PI3P appearance on ATG9A compartments with little PI, suggesting a lipid transfer-driven feedback loop supporting lipid transfer and ATG8 lipidation.
Data related to “Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy”
By onData related to "Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy"
RNA scope for ATP10b
By onProtocol for RNAscope® in situ hybridization on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit. Includes staining steps for dorsal striatum, sample preparation, and cryosectioning.
Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved TBM motif
By onInherited mutations in VPS35 and LRRK2 cause Rab GTPase hyperphosphorylation. TMEM55B on lysosomes interacts with RILPL1 through a specific motif, forming complexes with various proteins, revealing new insights into lysosomal function.
Neuromodulator control of energy reserves in dopaminergic neurons
By onNeurons, are vulnerable to metabolic changes. Dopamine helps regulate glycogen storage providing resilience to stress. Loss of this regulation can make dopamine neurons hypersensitive to fuel deprivation, potentially leading to neurodegeneration.
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g1)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
Integrated Representations of Threat and Controllability in the Lateral Frontal Pole
By onEmotions can strongly influence behavior, as seen in PD paradoxical kinesia. Here, we demonstrate that anticipating threats enhances motor accuracy through interactions between the amygdala and the anterior pole of the lateral prefrontal cortex.
L1-silenced iPSC line – Control (HS1_L1HS-CRISPRi_LacZ )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g2 )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_LacZ)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_g1)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
A single Citrobacter rodentium infection in Pink1 knockout and wild type mice leads to regional blood-brain-barrier perturbation and glial activation without dopamine neuron axon terminal loss
By onWe tested whether a gut infection causes blood-brain barrier permeability and brain inflammation. Results showed increased BBB permeability in specific brain regions at 26 days while tight-junction and dopamine related proteins remained unchanged
Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis
By onUsing single cell RNA sequencing, we demonstrate that LRRK2 G2019S is associated with an increased neutrophil presence in the colonic lamina propria during infection, Th17 skewing, upregulated Il17a, and greater colonic pathology.
