High-Yield Monocyte/Macrophage Differentiation from hiPSC
Output Details
Description
Here, a highly efficient method for differentiating monocytes/macrophages from hiPSC is described. The process utilizes commercially-available materials to derive CD34+ progenitor cells that are apically released from a hemogenic endothelium. Subsequently, the hemogenic endothelium gives rise to highly pure (>95%), CD34-CD14+ monocytes in 19-23 days and yields more monocytes by day 35 when compared to previous methods. These monocytes are further differentiated to macrophages after 7 days. The efficient workflow and increase in monocyte output boost feasibility for high throughput studies and enables clinical-scale iPSC-derived manufacturing processes.
Identifier (DOI)
10.17504/protocols.io.14egn37oql5d/v1
