Protocol Archive

This protocol details the primary neuron culture procedures from embryonic and postnatal mice.

These procedures are done in the context of calcium imaging experiments in non-human primates. The authors inject a solution containing AAV-GCaMP6 into the supplementary motor area (SMA) of the animal to be tested and then insert the GRIN lens into the injected area during the same session.

This protocol details ventral midbrain genomic PCR.

This protocol details the staining for free floating mouse brain sections.

This protocol details the immunofluorescence staining in mouse brain tissue sections.

Protocol for detecting pRab proteins in mouse brain tissue.

Protocol for performing ImmPRESS immunostaining with alkaline phosphatase labeling after BaseScope In Situ Hybridization mouse brain sections.

PET/CT and MRI for multimodal image compatibility.

This protocol describes the steps to use postmortem imaging to assess the accuracy of an implant by visualizing implant location with a small thermal ablation and comparing its coordinates to image-based pre-surgical planning.

This protocol details step by step how to use stereotaxic frame features to align (or coregister) monkey head CT and MR images in a clinical ROSA ONE Brain robot (website link), in preparation for electrode insertion planning.

EMG recordings is a common method to confirm implant location. In this protocol, the authors describe steps to perform EMG recordings during an internal capsule stimulator implant surgery.

Quality assessment for images obtained using 3D printed stereotaxic frame.

This protocol describes the procedure to quantify glutamate released from induced pluripotent stem cells derived cortical neurons (iPSC-CNs) cultured in adherent monolayer.

This series of protocols has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow: Note that the original QUINT workflow was generated by Yates and colleagues, and all credit for the development of these programs goes to that team.

A protocol for live-cell imaging of yeast cells expressing human PINK1 with GFP tag at the C-terminus as well as all the human TOM complex subunits (TOMs 5, 6, 7, 20, 22, 40 and 70).

This protocol describes how to create single-molecule antibody slides for fluorescence microscopy.

Protocol for imaging tetraspeck beads on glass coverslips.

This protocol details rating scale for Parkinsonian motor signs in macaques and other non‐human primates.

This protocol is for T cell differentiation from mice spleen tissue to investigate T cell function (including differentiation and proliferation) in vitro.

This is the protocol for the genetic modification of human induced pluripotent stem cells (hiPSCs) using lentiviral transduction method.