Protocol Archive

This protocol describes preparation of organotypic hemisphere cultures prepared from mouse brain and cultured as rollerdrum cultures on glass coverslips.

Protocol for preparing post-mortem tissue for intracellular NM quantification.

Quantification of area and optical density of intracellular neuromelanin with TruAI.

Designing and properly mounting FFPE sections on large-window CytAssist slides is important for downstream analysis and data quality. This protocol describes the key steps for this preparation.

This is the protocol to scan brain neuromelanin with reduced impact of the chemicals on their original color and intensity. The immunofluorescence staining protocol is designed to identify human A6, A9, and A10 cell clusters and alpha-synuclein pathology.

This protocol details acute extracellular multi-unit recordings and optotagging in mice.

This protocol describes an automatizable pipeline for cell counting based on fluorescent stainings with the help of CellPose. Additionally, it describes the entire generation process of plots that show the intensity of neuromelanin per cell based on bright-field images.

This protocol describes juxtacellular recordings in the VTA and LC of adult mice, involving anesthesia, precise brain targeting, and neuron labeling. Using specialized electrodes and a dual-channel amplifier, neuronal activity is recorded and modulated with current injections. Following recordings, brains are perfused, fixed, and sectioned for detailed morphological analysis.

The sucrose preference test assesses the animal’s interest in a sweet-tasting sucrose solution relative to unsweetened water.

Protocol for immunohistochemistry on rat brain cryosections.

Protocol for immunofluorescence on rat brain cryosections.

Protocol for quantifying TH+ fibers in rat striatum and substantia nigra pars reticulata (SNpr).

Protocol for Sholl analysis by Neurolucida software.

Protocol for constant potential amperometry recordings in vitro of evoked dopamine release in the rat striatum.

Protocol to assess motor coordination and balance by using an accelerating rod.

The open field test is a simple test to evaluate general motor and explorative behavior in rodents.

This protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).

Quantification of area and optical density of intracellular neuromelanin with Image J.

Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.

Quantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections.