Staining of RAW cells for RNA oxidation

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Description

Validation of RNA oxidation antibody in cultured RAW cells A. Treatment with RNAse or DNAse B. Isotype control or no primary antibody C. Preabsorption of antibody with nucleotide D. In vitro oxidation prior to staining (this does not notably increase signal)

Identifier (DOI)

10.17504/protocols.io.qpvonx76zl4o/v1

Discovery Team

Team Parisiadou

Program

Collaborative Research Network

Theme

PD Heterogeneity

Meet the Authors

Sabine Hilfiker

External Collaborator
Yahaira Naaldijk

External Collaborator
Divyangi (Divya) Kantak

External Collaborator

Staining of RAW cells for RNA oxidation

Output Details

Meet the Authors

Sabine Hilfiker

Yahaira Naaldijk

Divyangi (Divya) Kantak