Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
By onThis protocol describes the maintenance and expansion of iPSCs in the adherent culture via single-cell passaging.
Standard Operating Procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates
By onThis protocol details standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates.
Protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By onStep by step procedure for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice.
Rat brain processing for histological analyses
By onProtocol for rat brain processing for histological analyses.
Brain Slice Preparation for electrophysiology recording
By onThis protocol describes brain slice preparation for electrophysiology recording.
Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
By onThe results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.
Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”
By onThe files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.
Mouse Behavior – Open Field and T-Maze
By onThis protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
Scalable, flexible carbon fiber electrode thread arrays for three-dimensional probing of neurochemical activity in deep brain structures of rodents
By onThe authors' CFET array has the potential to unlock a wide range of applications, from uncovering the role of neuromodulators in synaptic plasticity, to addressing safety barriers in clinical translation toward diagnostic and adaptive treatment in PD
Live Imaging of Primary Mouse Neuron Cultures
By onThis protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging described involves labeling of dopamine neurons with a fluorescent DAT ligand and using virally encoded pHlourin sensors to measure vesicular release of neurotransmitter as the neurons are electrically stimulated. This technique was used in Jain et al., 2023 to compare vesicular release in neurons between various transgenic knockout mouse lines.
RNAScope in situ hybridization (ISH) multiplex fluorescent
By onInstructions to perform ISH using RNAscope kit from ACDBio company optimized for frozen mouse brain and nodose ganglia sections.
In Vivo Electrophysiology (Mouse)
By onThis protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.
Immunohistochemistry
By onThis protocol describes immunohistochemical staining of fixed brain sections.
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
By onThis protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.
Accelerated Ovarian Failure AOF mouse model: a model of transitional menopause
By onThe novel model of AOF successfully replicates the human peri- and post-menopause stages, including erratic estrous cycles and hormone fluctuations, followed by undetectable estrogen levels.
Maintenance of the recording chamber for non-human primate (Chamber cleaning and dura scraping)
By onThis protocol explains two procedures to maintain the recording chamber in nonhuman primates with craniotomies:(1) chamber cleaning and (2) dura scraping.
Datasets associated with “Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology”
By onDataset associated with the publication "Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology"
Handling and behavioral training protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By onThis protocol details the habituation of animals and behavioral training of animals for in vivo electrophysiology or optogenetic experiments.
Stereotactic Viral Injection into Subthalamic Nucleus in Mice
By onThis protocol describes the surgical procedure of injecting AAV viruses into the subthalamic nucleus of a mouse. It contains information regarding the stereotactic setup, surgery preparation, anesthesia, craniotomy, injection, and recovery.
