Preparation of midbrain slices for electrophysiology
By onProtocol for the preparation of midbrain slices for electrophysiology
Analyses of mitochondrial function in SNpc with the Seahorse XFe96
By onProtocol for analyses of mitochondrial function in SNpc with the Seahorse XFe96
Patch-clamp recordings of SNpc dopamine neurons from midbrain slices
By onProtocol for patch-clamp recordings of SNpc dopamine neurons from midbrain slices
Development of a simplified smell test to identify Parkinson’s disease using multiple cohorts, machine learning and item response theory
By onReduced olfaction is a common feature of typical Parkinson disease (PD) and dementia with Lewy bodies (DLB). We have created a simplified smell test based on seven specific odorants that can distinguish PD/DLB patients from healthy controls.
Leveraging animal models to understand non-motor symptoms of Parkinson’s disease
By onParkinson's disease diagnosis focuses on motor symptoms, but non-motor symptoms like cognitive issues and sleep disorders significantly affect patients. Research on non-motor signs in animals aims to improve understanding of the disease.
Astrocytic polyamine transport by ATP13A4 tunes excitatory synaptic transmission
By onPolyamines are crucial for brain function. ATP13A4 is the main polyamine transporter in astrocytes, impacting astrocyte morphology, synapse formation, and neurodevelopment. Mutations in ATP13A4 are linked to neurodevelopmental disorders.
Preparation of Acute Brain Slices
By onThis protocol describes the steps for preparing acute brain slices.
Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses I
By onThis dataset provides a unifying logic for how the developmental specification of diverse SNr neurons relates to the anatomical organization of basal ganglia circuits controlling specialized downstream brain regions.
Single nucleus RNA sequencing of SNr neurons
By onThese findings provide a unifying logic for how the developmental specification of diverse SNr neurons relates to the anatomical organization of basal ganglia circuits controlling specialized downstream brain regions.
pAAVDJ-CAG-FlpX-mKate2.0-N2cG
By onThe pAAV-CAG-fDIO-mKate2.0-N2cG vector was used as a helper virus in conjunction with a rabies virus to label presynaptic inputs to various regions in the brain with focus on SNr neurons.
Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons
By onThis study compared the efficacy and selectivity of 11 different AAV serotypes for the efficient and selective transduction of murine olfactory sensory neurons in vivo. The findings inform research approaches and gene therapy based initiatives.
Stereotaxic Surgery
By onThis protocol describes the steps for performing mouse stereotaxic surgery. It is applicable to intracranial injections of viruses and drugs.
Postsynaptic adaptations in direct pathway muscarinic M4-receptor signaling follow the temporal and regional pattern of dopaminergic degeneration
By onImbalances in dorsal striatum output in Parkinson's disease are driven by dopamine loss and disrupted acetylcholine signaling. These changes occur in response to dopamine loss, affecting M4 receptors in striatal neurons, crucial for PD progression.
6-OHDA mouse model of Parkinson’s disease
By onProtocol for creating a Parkinson's disease mouse model using 6-OHDA involves injecting different doses into the brain to induce varying levels of dopaminergic damage.
Levodopa treatment for low-dose 6-OHDA-treated mice
By onThis protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Electrophysiology and 2-photon imaging of Ca+2-transients
By onProtocol for imaging dendritic calcium transients using 2-photon microscopy with a Ti:Sapphire laser. Fluorescence collected through objective, dichroic mirror, and filters, then detected by a GaAsP PMT. Output digitized for analysis.
Ex Vivo Electrophysiology – Ford Lab
By onThis protocol describes the steps to perform whole-cell electrophysiology recordings in acute brain slices.
