This protocol describes stereotaxic delivery of 6-hydroxydopamine (6-OHDA) to generate either a mild medial forebrain bundle (MFB) lesion model in 3- to 5-month-old wild-type C57BL/6J mice.
The protocol describes in detail how to induce GCase loss of function in mice using CBE treatment. Mice exhibit severe motor symptoms accompanied by neuroinflammation after four weeks of daily CBE injections using this protocol.