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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Lab Resource
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
This construct links GCaMP8s to Synaptophysin and directs the fusion protein to presynaptic sites. It also produces a cytoplasmic red fluorescence protein (mRuby) via the self-splicing peptide 2A. It is mainly used to longitudinally image synaptic activity with 2-Photon at 980nm and visualize axons at 1080nm in vivo.
Teams
Lab Resource
pAAV_Cbh_DIO_hTyr
Cre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. The CbH promoter is a strong, pan-neuronal, non-silencable promoter.
Teams
Lab Resource
pAAV_Ef1a_DIO_hTyrHA_minBack
Cre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. Expression under strong Ef1a promoter.
Teams
Lab Resource
pAAV_Ef1a_DIO_hTyrNull_HATag
Cre-dependent expression of a truncated human tyrosinase is inactive and does not lead to the accumulation of neuromelanin in catecholaminergic neurons (control virus). Strong expression of Ef1a promoter, HA tag for histological verification of expression.
Teams
Lab Resource
pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE
This links GCaMP8f with a ribosome-tethered nanobody that restricts GCaMP to the soma of neurons. Particularly useful in very dense nuclei with strong neuropil, which can make post-hoc cell segmentation (i.e., independent component analysis (ICA) or constrained nonnegative matrix factorization (CMNF)) difficult.
Teams
Lab Resource
pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack
Bright green (mNeon) and red (mRuby) fluorescence proteins are expressed, with the former being cytoplasmic and the latter membrane-targeted. This expression is ideal for tracking long diffuse axons in cortical structures in vivo or for slice histology and cleared brain tissues post-mortem.
Teams
Lab Resource
pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA
A viral construct designed to express GCaMP6m pan-neuronally and express a red fluorophore (mRuby) in the nucleus of cortical interneurons. This construct is ideal for dual-imaging experiments to capture activity of two neuronal populations and to identify interneurons with red fluorescence in parallel or post-mortem.
Teams
Lab Resource
pAAV_Cbh_DIO_hTyrNull_HATag
Cre-dependent expression of a truncated human tyrosinase is inactive and does not lead to the accumulation of neuromelanin in catecholaminergic neurons (control virus). Strong expression of CbH promoter that is non-silencenable, HA tag for histological verification of expression.
Teams