pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL > AEVA)
By onpPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA) plasmid
Progressive noradrenergic degeneration and motor cortical dysfunction in Parkinson’s disease
By onThe article discusses clinical and preclinical studies that support the critical rolLC-NE neurodegeneration and motor cortical dysfunction in both motor and nonmotor deficits in Parkinsonian states.
pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI > ATFA)
By onpPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA) plasmid
GRAB Acetylcholine (ACh) sensor imaging in ex vivo mouse striatal slices
By onProtocol for imaging GRAB Acetylcholine (ACh) sensor in mouse striatal slices and measuring changes in fluorescence signals due to ACh release is described.
Sub-second characterization of locomotor activities of mouse models of Parkinsonism
By onThis article documents robust changes in the velocity, usage, and temporal organization of behavioral modules and their responsiveness to dopaminergic treatment under the Parkinsonian state.
Dopamine and cortical iPSC-derived neurons with different Parkinsonian mutations show variation in lysosomal and mitochondrial dysfunction: implications for protein deposition versus selective cell loss
By onComparison of dopamine neurons identified lysosomal and mitochondrial dysfunction, as well as increased tau deposition, alpha-synuclein phosphorylation and decreased glucocerebrosidase activity.
Transgenic A53T mice have astrocytic a-synuclein aggregates in dopamine and striatal regions
By onUsing fluorescent immunohistochemistry, astrocyte subtypes were identified and aSyn burden quantified.
Unilateral 6-hydroxydopamine lesion mouse model of Parkinson’s disease
By onTo generate a mouse model of Parkinson's disease, unilateral injection of 6-OHDA(6-hydroxydopamine) into the medial forebrain bundle (MFB) was performed. Three to four weeks later, a drug-free cylinder test was used to assess the extent of lesion.
⍺-Synuclein levels in Parkinson’s disease – Cell types and forms that contribute to pathogenesis
By onParkinson's disease is characterized by dopamine neuron loss and ⍺-synuclein aggregations in neurons. Research focuses on understanding the levels, modifications, and impact of ⍺-synuclein in brain cells, including non-neuronal cells.
Ex vivo imaging of dendritic calcium transients and spines with two-photon laser scanning microscopy (2PLSM)
By onThis is an adapted protocol for imaging dendritic Ca2+ transients and dendritic spines of striatal spiny projection neurons (SPNs) in ex vivo brain slices with two-photon laser scanning microscopy (2PLSM).
Rating of levodopa-induced dyskinesia in 6-OHDA lesioned mice
By onThis protocol describes scoring of abnormal involuntary movements (AIMs), the behavioral manifestation of levodopa-induced dyskinesia, in 6-OHDA lesioned mice.
Sparse labeling-enabled confocal imaging of dendritic spines followed by analysis
By onThis protocol describes procedures starting from sparsely labelling neurons to analyzing their dendritic spines, including stereotaxic injection, brain perfusion and fixation, sectioning and mounting, confocal imaging and spine reconstruction.
Ex vivo imaging of genetically encoded sensors with two-photon laser scanning microscopy (2PLSM)
By onThis protocol has been adapted for imaging genetically encoded sensors (e.g. GRABACh3.0) in ex vivo brain slices with two-photon laser scanning microscopy (2PLSM).
Python script for analyzing Prairie View 5-generated .csv files of somatic excitability data
By onScript used to analyze somatic excitability data in Figures 1, 3, 6, 7 and 8 of Zhai et al 2025.
Zhai et al. 2025 Data files
By onData used to generate figures in the manuscript "State-dependent modulation of spiny projection neurons controls levodopa-induced dyskinesia in a mouse model of Parkinson's disease" by Zhai et al.
Targeting mitophagy in neurodegenerative diseases
By onMitochondrial dysfunction is key in neurodegenerative diseases like Parkinson's and Alzheimer's. Enhancing mitophagy could be a new therapeutic approach, with USP30 inhibitors and PINK1 activators in phase I trials for potential disease modification.
Python script for analyzing Prairie View 5-generated .csv files of BOT imaging data
By onPython script for analyzing Prairie View 5-generated .csv files of BOT imaging data in Figures 5 and S2 of Zhai et al 2025.
Python script for analyzing Prairie View 5-generated .csv files of dendritic excitability data
By onPython script for analyzing Prairie View 5-generated .csv files of dendritic excitability data in Figures 1, 3, 6, 7 in Zhai et al 2025.
Mediterranean Diet Adherence, Gut Microbiota and Parkinson’s Disease: A Systematic Review
By onHigh adherence to the Mediterranean diet may improve cognitive function and gastrointestinal symptoms in Parkinson's disease patients, possibly due to changes in gut microbiota. More research is needed to understand its effects on motor symptoms.
Consensus Guidance for Genetic Counseling in GBA1 Variants: A Focus on Parkinson’s Disease
By onThis review discusses the link between *GBA1* and PD, testing practicalities, and counseling approaches.
