Prospects for Disease Slowing in Parkinson Disease
By onDespite advancements in understanding PD pathogenesis and developing targeted therapies, no treatment has proven effective in modifying the disease. This review discusses current pharmacological interventions in clinical trials for PD.
Presynapses are mitophagy pit stops that prevent axon degeneration
By onDefective neuronal mitophagy is linked to PD. Damaged axonal mitochondria accumulate at presynapses, sites of mitophagy initiation and autophagosome formation. of receptor mediated mitophagy with Roxadustat, reveals a potential therapeutic avenue.
README Guide for Code
By onThe purpose of this document is to provide guidance on how to write README files for code.
Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclasses
By onOur findings in this paper provide a unifying logic for how the developmental specification of diverse SNr neurons relates to the anatomical organization of basal ganglia circuits controlling specialized downstream brain regions.
Brain infiltrating leukocytes (BILs) extraction
By onThis protocol details the extraction of brain infiltrating leukocytes (BILs).
Mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay
By onThis protocol details the mitochondrial antigen presentation (MitAP) to primary 2C CD8 T (proliferation and suppression) assay in vivo
Similarities and differences between nigral and enteric dopaminergic neurons unravel distinctive involvement in Parkinson’s disease
By onParkinson's disease affects both midbrain and enteric nervous system dopaminergic neurons. Shared factors and properties control their evolution, with potential for novel neurotrophic factors to be used as protection against PD symptoms in the ENS.
Interactions of dopamine, iron, and alpha-synuclein linked to dopaminergic neuron vulnerability in Parkinson’s disease and Neurodegeneration with Brain Iron Accumulation disorders
By onDopamine, alpha-syn, and iron dysregulation contribute to vulnerability of substantia nigra dopaminergic neurons in Parkinson's and related disorders. Understanding their interplay is crucial for developing treatments to prevent disease progression.
Gut microbiome-mediated regulation of neuroinflammation
By onGut microbiome influences neuroinflammatory disease through pathways, including metabolite production, immune cell trafficking, and vagus nerve. Research on gut-brain axis has promising implications for understanding and treating neuroinflammation.
Single nucleus transcriptomic dataset of AAV-infected mouse OE
By onSingle nucleus sequencing dataset from mouse murine olfactory epithelium exposed to 4 AAV serotypes via nasal lavage.The goal was to identify the best candidate for transduction of olfactory sensory neurons. Code is included with the dataset.
Computational analysis for “Comparative Analysis of AAV Serotypes for the Transduction of Olfactory Sensory Neurons” by Belfort and Jia et al. 2024
By onCode for snRNAseq dataset analysis of AAV-infected mouse olfactory epithelium. This code accompanies the manuscript: Comparative Analysis of AAV Serotypes for the Transduction of Olfactory Sensory Neurons by Belfort, Jia et al. 2024.
Comparative Analysis of AAV Serotypes for Transduction of Olfactory Sensory Neurons
By onThis study compared the efficacy and selectivity of 11 different AAV serotypes for the efficient and selective transduction of murine olfactory sensory neurons in vivo. The findings inform research approaches and gene therapy based initiatives.
Astrocyte generation
By onA protocol for generating astrocytes from cortical neural progenitor cells using a commercial astrocyte media (AM) is described.
Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human-Induced Neurons
By onThe endolysosomal system regulates plasma membrane protein homeostasis and is crucial for neuronal function and implicated in Parkinson's disease. We present a proteomics tool to analyze the endolysosomal system in human induced cortical-like neurons
H9 ES AAVS1-NGN2 3xFLAG-EEA1 TMEM192-3xHA
By onES cells were modified to create iNeurons expressing EEA1 with a 3xFLAG tag and TMEM192 with 3xHA tag. NEUROG2 was introduced at the AAVS1 locus using CRISPR/Cas9. The cells are derived from human embryonic stem cells at the blastocyst stage.
Microscopy dataset for: Comparative Analysis of AAV Serotypes for Transduction of Olfactory Sensory Neurons
By onImaging dataset for the manuscript "Comparative Analysis of AAV Serotypes for Transduction of Olfactory Sensory Neurons ", by Belfort, Jia et al., 2024.
Intracranial injections of viral vectors in mouse striatum
By onProtocol for intracranial viral vector injections in dorsal or ventral striatum in mice is outlined. Steps are generalizable, but require optimization for injection coordinates and virus concentration.
Making Ca2+ – selective microelectrodes
By onProtocol for making Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices.
Abnormal hyperactivity of specific striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering
By onL-DOPA-induced dyskinesia is a common issue in Parkinson's disease due to dopamine therapy. These findings show that ensembles of behavior-encoding D1- and D2-SPNs form new combinations of hyperactive neurons mediating specific dyskinetic movements.
Custom Python and MATLAB scripts related to Stedehouder & Roberts (2024) “Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes”
By onScripts by Jeffrey Stedehouder, Stefania Vietti-Michelina & Professor Kevin McGerty to analyse and plot FCV, patch-clamp, and GRAB sensor imaging experiments in ex vivo mouse brain slices, and to model the distance between cell types.
