NanoString nCounter Gene Expression CodeSet RNA Hybridization Protocol
By onNanoString's technology uses molecular barcodes for digital detection in highly multiplexed analysis. Reporter CodeSet and Capture ProbeSet bind to targets, excess probes are removed, and barcodes are counted on nCounter™ systems.
A Protocol for Neuralized Murine Olfactory Organoids
By onThis manuscript describes the methodology to generate olfactory organoids from olfactory epithelium isolated from adult mice. These organoids contain both neuronal and non-neuronal cell types and have been kept in culture for up to 3 weeks.
Hydrophobic tails enable diverse functions of the extracellular chaperone clusterin
By onStructure-based functional analysis of the medically important extracellular chaperone and apolipoprotein Clusterin, revealed two hydrophobic tails key for chaperone function, membrane receptor binding, cellular uptake, and apolipoprotein formation.
Thalamic integration of basal ganglia and cerebellar circuits during motor learning
By onBasal ganglia and cerebellum are vital for motor control and learning. Our works shows thalamic neurons integrate signals from both regions, and show distinct movement-related activity which play a key role in motor learning and control in mice.
Viral overexpression of human alpha-synuclein in mouse substantia nigra dopamine neurons results in hyperdopaminergia but no neurodegeneration
By onNovel viral vector for α-syn overexpression, AAV-DIO-hASYNWT, can be expressed selectively in neuronal populations based on Cre. Overexpression in DANs did not cause degeneration. AAV-DIO-hASYNWT increased DA levels and locomotor hyperactivity.
ZSGreen x TH Colocalization Analysis
By onColocalization analysis of TH and ZSGreen using immunohistochemistry
Single cell long read whole genome sequencing reveals somatic transposon activity in human brain
By onSingle cell long-read sequencing uncovers novel dynamics in three brains, shedding light on genomic variability. It reveals brain-specific transposable element activity, offering crucial insights into individual cell genomes.
A data repository for the study of Alpha-synuclein aggregates trigger anti-viral immune pathways and RNA editing in human astrocytes
By onA data repository for the study of Alpha-synuclein aggregates trigger anti-viral immune pathways and RNA editing in human astrocytes
The mitochondrial unfolded protein response promotes senescence in human microglia by increasing S-adenosylmethionine availability for polyamine synthesis
By onMitochondria have a response to stress called UPRmt, aiding proteostasis. In humans, UPRmt affects microglia, causing metabolic changes and disrupting communication, leading to dysfunction and inflammation, impacting brain aging and disease.
Datasets and Key Resources Table used in Stedehouder & Roberts (2024) “Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes”
By onThis repository contains a Key Resources Table with details on lab materials and the persistent identifiers for protocols and code used and generated in this study; and tabular datasets shown in main and supplementary figures.
Additional data repository for the study of Alpha-synuclein aggregates trigger anti-viral immune pathways and RNA editing in human astrocytes
By onZip file 1: astrocytes calcium data measured using Fura 2 Zip file2: astrocytes ROS measured using DHE (Dihydroethidium) Zip file 3: astrocytes cell death measured using Sytox green
Raw files for Part1: Endogenous LRRK2 and PINK1 function in a convergent neuroprotective ciliogenesis pathway in the brain
By onAll data presented in Figure 1, 3, 4 and 5
The pyrethroid insecticide deltamethrin disrupts neuropeptide and monoamine signaling pathways in the gastrointestinal tract
By onEnteroendocrine cells in the intestine produce hormones affecting motility. Pyrethroids, common insecticides, impact these cells, leading to altered hormone release and intestinal motility, potentially contributing to various diseases.
Raw files for Part3: Endogenous LRRK2 and PINK1 function in a convergent neuroprotective ciliogenesis pathway in the brain
By onAll data presented in Figure 6
Raw files for Part2: Endogenous LRRK2 and PINK1 function in a convergent neuroprotective ciliogenesis pathway in the brain
By onAll data presented in Figure 2
A Vulnerable Subtype of Dopaminergic Neurons Drives Early Motor Deficits in Parkinson’s Disease
By onThis study uncovers the existence of a selectively vulnerable subtype of dopaminergic neurons (DANs) that is sufficient to drive early motor symptoms in Parkinson’s disease.
State-dependent modulation of spiny projection neurons controls levodopa-induced dyskinesia in a mouse model of Parkinson’s disease
By onLID is poorly understood, our research on dyskinetic mice show SPNs' properties oscillate during LID and are influenced by dopamine and acetylcholine signaling. Targeting M1 receptors in iSPNs reduced LID severity and improved levodopa effects.
pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI > ATFA)
By onpPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA) plasmid
