Confocal imaging of transcriptional crosstalk between AAV genomes carrying different enhancers in the mouse brain
By onA data collection of images of brains of mice transduced with viral vectors carrying different regulatory elements and reporters to study transcriptional crosstalk between AAV genomes
Data associated with “Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables delivery of large genetic cargo”
By onTabular data associated with the publication "Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables delivery of large genetic cargo"
Mouse EEG implantation and monitoring
By onProtocol for EEG implantation surgery and EEG data collection for mouse models
Systemic AAV administration through retro-orbital injection
By onProtocol for administering an AAV systemically through injection into the retro-orbital sinus of mouse
AAV-Zombie on cultured cells
By onProtocol for detecting AAV genomes and concatemers in cultured cells by the Zombie method
Immunohistochemistry (IHC) on mouse brain slices
By onProtocol for immunohistochemical localization and visualization of proteins in mouse brain tissue (free-floating or slide-mounted)
Absolute quantification of AAV genomes from total DNA with digital droplet PCR (ddPCR)
By onProtocol for absolute measurement of AAV genome number from total DNA samples extracted from animal tissues
Controls, imaging, and analysis of AAV-Zombie and SpECTr experiments
By onProtocol describing best practices for designing, imaging, and analyzing AAV-Zombie and SpECTr experiments
AAV-Zombie on tissue sections
By onProtocol for detecting AAV genomes and concatemers in tissue by the Zombie method
RGS10 Attenuates Systemic Immune Dysregulation Induced by Chronic Inflammatory Stress
By onRGS10, a key regulator of immune cells, is decreased in individuals with Parkinson's Disease. Its deficiency, especially in males, leads to inflammatory responses in peripheral and CNS immune cells, suggesting a role in immune dysregulation in PD.
Assessment of mouse motor behaviour via open field test, narrowing beam assay, inverted wire hang, and gait analysis
By onProtocol for assessing mouse behaviour on 4 tasks: open field test, narrowing beam assay, inverted wire hang, and gait analysis
NanoString nCounter Gene Expression CodeSet RNA Hybridization Protocol
By onNanoString's technology uses molecular barcodes for digital detection in highly multiplexed analysis. Reporter CodeSet and Capture ProbeSet bind to targets, excess probes are removed, and barcodes are counted on nCounter™ systems.
A Protocol for Neuralized Murine Olfactory Organoids
By onThis manuscript describes the methodology to generate olfactory organoids from olfactory epithelium isolated from adult mice. These organoids contain both neuronal and non-neuronal cell types and have been kept in culture for up to 3 weeks.
Hydrophobic tails enable diverse functions of the extracellular chaperone clusterin
By onStructure-based functional analysis of the medically important extracellular chaperone and apolipoprotein Clusterin, revealed two hydrophobic tails key for chaperone function, membrane receptor binding, cellular uptake, and apolipoprotein formation.
Thalamic integration of basal ganglia and cerebellar circuits during motor learning
By onBasal ganglia and cerebellum are vital for motor control and learning. Our works shows thalamic neurons integrate signals from both regions, and show distinct movement-related activity which play a key role in motor learning and control in mice.
Viral overexpression of human alpha-synuclein in mouse substantia nigra dopamine neurons results in hyperdopaminergia but no neurodegeneration
By onNovel viral vector for α-syn overexpression, AAV-DIO-hASYNWT, can be expressed selectively in neuronal populations based on Cre. Overexpression in DANs did not cause degeneration. AAV-DIO-hASYNWT increased DA levels and locomotor hyperactivity.
ZSGreen x TH Colocalization Analysis
By onColocalization analysis of TH and ZSGreen using immunohistochemistry
