Unilateral 6-hydroxydopamine lesion mouse model of Parkinson’s disease
By onTo generate a mouse model of Parkinson's disease, unilateral injection of 6-OHDA(6-hydroxydopamine) into the medial forebrain bundle (MFB) was performed. Three to four weeks later, a drug-free cylinder test was used to assess the extent of lesion.
⍺-Synuclein levels in Parkinson’s disease – Cell types and forms that contribute to pathogenesis
By onParkinson's disease is characterized by dopamine neuron loss and ⍺-synuclein aggregations in neurons. Research focuses on understanding the levels, modifications, and impact of ⍺-synuclein in brain cells, including non-neuronal cells.
Ex vivo imaging of dendritic calcium transients and spines with two-photon laser scanning microscopy (2PLSM)
By onThis is an adapted protocol for imaging dendritic Ca2+ transients and dendritic spines of striatal spiny projection neurons (SPNs) in ex vivo brain slices with two-photon laser scanning microscopy (2PLSM).
Rating of levodopa-induced dyskinesia in 6-OHDA lesioned mice
By onThis protocol describes scoring of abnormal involuntary movements (AIMs), the behavioral manifestation of levodopa-induced dyskinesia, in 6-OHDA lesioned mice.
Sparse labeling-enabled confocal imaging of dendritic spines followed by analysis
By onThis protocol describes procedures starting from sparsely labelling neurons to analyzing their dendritic spines, including stereotaxic injection, brain perfusion and fixation, sectioning and mounting, confocal imaging and spine reconstruction.
Ex vivo imaging of genetically encoded sensors with two-photon laser scanning microscopy (2PLSM)
By onThis protocol has been adapted for imaging genetically encoded sensors (e.g. GRABACh3.0) in ex vivo brain slices with two-photon laser scanning microscopy (2PLSM).
Python script for analyzing Prairie View 5-generated .csv files of somatic excitability data
By onScript used to analyze somatic excitability data in Figures 1, 3, 6, 7 and 8 of Zhai et al 2025.
Zhai et al. 2025 Data files
By onData used to generate figures in the manuscript "State-dependent modulation of spiny projection neurons controls levodopa-induced dyskinesia in a mouse model of Parkinson's disease" by Zhai et al.
Targeting mitophagy in neurodegenerative diseases
By onMitochondrial dysfunction is key in neurodegenerative diseases like Parkinson's and Alzheimer's. Enhancing mitophagy could be a new therapeutic approach, with USP30 inhibitors and PINK1 activators in phase I trials for potential disease modification.
Python script for analyzing Prairie View 5-generated .csv files of BOT imaging data
By onPython script for analyzing Prairie View 5-generated .csv files of BOT imaging data in Figures 5 and S2 of Zhai et al 2025.
Python script for analyzing Prairie View 5-generated .csv files of dendritic excitability data
By onPython script for analyzing Prairie View 5-generated .csv files of dendritic excitability data in Figures 1, 3, 6, 7 in Zhai et al 2025.
Mediterranean Diet Adherence, Gut Microbiota and Parkinson’s Disease: A Systematic Review
By onHigh adherence to the Mediterranean diet may improve cognitive function and gastrointestinal symptoms in Parkinson's disease patients, possibly due to changes in gut microbiota. More research is needed to understand its effects on motor symptoms.
Consensus Guidance for Genetic Counseling in GBA1 Variants: A Focus on Parkinson’s Disease
By onThis review discusses the link between *GBA1* and PD, testing practicalities, and counseling approaches.
Anxa1+ dopamine neuron vulnerability defines prodromal Parkinson’s disease bradykinesia and procedural motor learning impairment
By onVulnerable Anxa1-expressing DANs are reduced in prodromal PD, affecting movement and procedural learning. Their dysfunction explains early PD symptoms like bradykinesia and motor impairments.
Glycolipids in Parkinson’s disease: beyond neuronal function
By onGlycolipid balance is crucial for normal body function and its disruption can cause diseases like Parkinson's. Understanding glycolipid pathways can help develop treatments for neurodegenerative diseases like PD.
Data and code for “The gut microbiome promotes mitochondrial respiration in the brain of a Parkinson’s disease mouse model””
By onProcessed data and code for analysis and plotting for the publication "The gut microbiome promotes mitochondrial respiration in the brain of a Parkinson’s disease mouse model"
The gut microbiome promotes mitochondrial respiration in the brain of a Parkinson’s disease mouse model
By onIn this paper, the authors find that the gut microbiome increases mitochondrial respiration and oxidative stress in the brain, which enhances motor symptoms in a mouse model of PD.
Protocol for isolating mitochondria from mouse striatum
By onProtocol used in the Mazmanian lab to isolate striatal mitochondria from mouse for subsequent Seahorse and other analyses
Protocol for mouse striatal tissue dissection, mitochondrial protein extraction, and western blot analysis
By onProtocol used in the Mazmanian lab for preparation and analysis of mitochondrial proteins from the striatum of mouse.
Protocol for quantitative bulk transcriptomics of striatal tissue
By onProtocol used in the Mazmanian lab for quantitative bulk transcriptomics of mouse striatum tissue
