Sparse labeling-enabled confocal imaging of dendritic spines followed by analysis

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Description

This protocol describes all the procedures starting from sparsely labelling neurons to analyzing their dendritic spines, including stereotaxic viral injection, brain perfusion and fixation, brain sectioning and mounting, confocal imaging and spine reconstruction using Imaris software.

Identifier (DOI)

10.17504/protocols.io.bp2l6dn7rvqe/v1

Tags

AAV (Adenoassociated Virus)
Confocal microscopy
Ex Vivo
Image analysis
In Vivo
Microscopy - optical
Mouse
Perfusion
Sectioning

Team

Team Surmeier

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Qiaoling Cui, PhD

Key Personnel: Team Surmeier,

Northwestern University

Sparse labeling-enabled confocal imaging of dendritic spines followed by analysis

Output Details

Meet the Authors

Qiaoling Cui, PhD

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