Immunohistochemistry (IHC) on mouse brain slices

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Description

This protocol provides a starting point for immunohistochemical localization and visualization of proteins in mouse brain tissue. This can be useful for marking cell types of interest or investigating the effect of experimental manipulations on protein expression and/or localization. This protocol is compatible with free-floating tissue or tissue that has already been mounted onto a slide.

Identifier (DOI)

10.17504/protocols.io.5qpvokmq7l4o/v1

Tags

Immunohistochemistry

Team

Team Gradinaru

Program

Collaborative Research Network

Theme

Circuitry and Brain-Body Interactions

Meet the Authors

Gerard Coughlin, BSc

Key Personnel: Team Gradinaru,

California Institute of Technology

Immunohistochemistry (IHC) on mouse brain slices

Output Details

Meet the Authors

Gerard Coughlin, BSc

Related Research

Histology Albarran et al 2025

DAB ANTIBODY (IHC) STAINING PROTOCOL

Immunohistochemistry