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Immunohistochemistry using paraffin embedded tissue
Output Details
Description
This protocol describes how to stain paraffin wax embedded sections of tissue for alpha-synuclein by immunofluorescence or DAB/peroxidase immunohistochemistry and is based on Hurley et al., 2013 (https://doi.org/10.1093/brain/awt134).
The protocol works for many other antibodies and the only change necessary for most antibodies is determining the optimal dilution of the primary antibody and the best type of antigen-retrieval to use.
Identifier (DOI)
10.17504/protocols.io.eq2ly72ywlx9/v1