Making Ca2+ – selective microelectrodes

By Jeffrey Stedehouder, Dorly Verdier, Arlette Kolta and Stephanie Cragg, PhD
View Published Protocol

Output Details

Description
The following protocol describes how to make Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices. Glass micropipettes can be precisely (µm precision) located at/near a particular brain region/cell type of interest to measure rapid changes in Ca2+ concentrations.
Identifier (DOI)
10.17504/protocols.io.yxmvmeeqog3p/v1
Tags
  • Calcium
  • Equipment
  • Ex Vivo
  • Mouse
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes

The authors found very rapid modulation of dopamine release at sub-second timescales, mediated by changes to acetylcholine signalling, and a surprising cell-to-cell “satellite” configuration of astrocytes next to striatal cholinergic…

View Article

Feed-forward metabotropic signaling by Cav1 Ca2+ channels supports pacemaking in pedunculopontine cholinergic neurons

Cholinergic neurons (CNs) in the pedunculopontine nucleus (PPN) are lost in the course of Parkinson’s disease. PPN CNs have a distinctive physiological phenotype that shares only some of the features of other selectively vulnerable neurons in PD.

View Article

Inhibition of striatal dopamine release by the L-type calcium channel inhibitor isradipine co-varies with risk factors for Parkinson’s

This data show that LTCC function in DA axons, and isradipine effect, are locally governed and suggest they vary in a manner that in turn might impact on, or reflect, the cellular stress that leads to parkinsonian degeneration.

View Article
View More Outputs