pCAG/VPS34(R561D|R566D|K567D|K568D)
By onPlasmid for bacterial expression of VPS34(R561D|R566D|K567D|K568D)
Steered MD simulations of the inactive-active transition
By onProtocol outlines simulations of PI3KC3-C1 complex transitioning from inactive to active state with RAB1A on a lipid membrane using steered molecular dynamics.
Molecular Dynamics Simulations of Active, Membrane-Engaged Complex
By onThe protocol describes molecular dynamics simulations of PI3KC3-C1 complex with RAB1A on a lipid membrane for studying protein interactions.
Image Processing and 3D Reconstruction
By onThis workflow was used to analyze a Krios dataset of the PI3KC3-C1/RAB1A Complex and generate a reconstruction of three distinct conformational states of the VPS34 lipid kinase domain.
Unbiased MD simulations of POPI entry into VPS34KD
By onThis protocol details molecular dynamics simulations of the kinase domain of VPS34 in a membrane containing 15% of POPI lipids and analysis of POPI entry into the catalytic site.
Costa Lab Nanoject Virus Injections
By onThis is the standard stereotaxic/Nanoject injection mouse surgery protocol for the Costa lab at Columbia University.
Open Field Behavior in Mice-Ding Lab
By onThe open field test is widely used to assess spontaneous locomotor activity, exploratory behavior, and anxiety-like responses in rodents.
Chronic Cranial Window and Micro-Optical Probe Implantation in Mice
By onMonitoring neuronal structure and activity in behaving animals helps understand movement control. Surgical procedures like chronic cranial window and micro-optical probe implantation allow optical monitoring of neural circuits.
