Drugs and treatment dosing
By onDrugs and treatment dosing protocol related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
LC-MS/MS Analysis
By onLC-MS/MS Analysis related to LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
Lipidomic analysis in HeLa cells
By onA protocol for the lipidomic analysis in HeLa cells is described.
Lipidomic analysis in HeLa cells for HexCer levels
By onA protocol for the lipidomics analysis in HeLa cells for HexCer levels is described.
Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
By onSynaptic dysfunctions manifest early in Parkinson's disease. Research on LRRK2 mutations, using advanced genetic models, reveals their effects on neuronal synapses, primarily impacted by the disease, offering insights into potential early therapies.
LRRK2 alters the composition and organization of active zone release sites in dopaminergic striatal synaptosomes
By onConfocal images and analysis overview
TH fibers and dopamine vesicle clustering alterations
By onTh fibers and dopamine vesicle clustering alterations in Lrrk2WT and Lrrk2G2019S mice
LRRK2 is expressed in dopamine neurons and is enriched in the vulnerable subpopulation
By onConfocal images from Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
LRRK2 disrupts the number and composition of active zone release sites in vulnerable dopamine axons
By onStriatal brain sections were stained with antibodies against bassoon to analyze the number and organization of active zone sites in Aldh1a1+ (EYFP+) or Aldh1a1- (mCherry+) axons from the same mouse
Immunofluorescence and confocal imaging in brain sections and image analysis
By onImmunofluorescence and confocal imaging in brain sections and image analysis for Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Axonal proteome analysis and Western Blot via APEX2 labeling
By onAxonal proteome analysis and western blot via APEX2 labeling related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
SIM imaging and analysis for active zone release sites in vulnerable dopamine axons
By onStereotactic injections and viruses described and SIM imaging and analysis
Liquid Chromatography-Mass Spectrometry (LCMS) with striatal synaptosomes
By onLiquid Chromatography-Mass Spectrometry (LC-MS) with striatal synaptosomes assay related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons preprint
Western blot and Phostag gels
By onWestern blot and Phostag gels protocol related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Proteomics for STING KO and STINGRRAA RAW 264.7 macrophages
By onUsing immunopeptidomics analysis we showed that STING regulates the repertoire of peptides displayed at the cell surface of macrophages during inflammation, highlighting a potential role in immunosurveillance and potential targets for therapy
Striatal synaptosome preparation for imaging
By onStriatal synaptosome imaging and analysis protocols in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Experiments in HEK-293 cells
By onProtocols related to experiments in HEK-293 cells in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons preprint
Custom scripts related to Graziano, M. et al. (2025) “A molecular atlas of cell-type specific signatures in the parkinsonian striatum”
By onScripts by Graziano and Masarapu aid in analyzing single-nucleus RNA sequencing data from Parkinson's Disease striatum, mapping vulnerabilities, and inferring cell trajectories. Giacomello Lab scripts are available on GitHub.
Code used in “Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis”
By onUsing scRNAseq and flow cytometry, we demonstrated that the Lrrk2-G2019S mutation is associated with an increased neutrophil presence in the colonic lamina propria, Th17 skewing, upregulated Il17a, and greater colonic pathology during infection.
