Lewy body diseases and the gut
By onGI involvement in Lewy body diseases may start with ⍺-synuclein in the gut spreading to the brain. Gut microbiome, immune system, and toxins play roles. These connections could lead to new therapies targeting the gut-brain axis for disease treatment.
Tamoxifen Administration in Transgenic Mice-Ding Lab
By onThis protocol describes the procedure for inducing CreER-mediated recombination at loxP sites in transgenic mice through the administration of tamoxifen or 4-hydroxytamoxifen (4-OHT).
RNA In situ hybridization (ISH) using RNAscope®
By onThis protocol outlines the procedures for RNA in situ hybridization to simultaneously detect up to three targets using the RNAscope® Multiplex Fluorescent v2 assay (ACD).
Head-fixed Forelimb Reaching Task in Mice
By onThis protocol describes a head-fixed forelimb reaching task used to study motor learning and memory in mice suitable for recording neuronal activity concurrently.
α-Synuclein aggregates inhibit ESCRT-III through sequestration and collateral degradation
By onα-Synuclein aggregates deplete ESCRT-III by sequestration and "collateral degradation," impeding endolysosomal repair. This allows endocytosed fibrils to leak into the cytoplasm, triggering a toxic cycle of aggregation and endolysosomal dysfunction.
Measuring lysosomal exocytosis by Flow cytometry
By onFlow cytometry can be used to measure lysosomal exocytosis by analyzing changes in fluorescence intensity of lysosomal markers in cells, providing a protocol for studying this cellular process.
GPNMB is a biomarker for lysosomal dysfunction and is secreted via LRRK2-modulated lysosomal exocytosis
By on*GPNMB* is linked to Parkinson's Disease as a risk factor, affecting its transcription and protein levels in CSF. Macrophages secrete GPNMB in response to lysosomal stress through lysosomal exocytosis, with LRRK2 influencing its secretion.
Lysosomal Glucocerebrosidase is needed for ciliary Hedgehog signaling: A convergent pathway to Parkinson’s disease
By onMutations in LRRK2 and GBA1 are common in familial Parkinson’s disease. These mutations inhibit primary cilia formation, blocking Hedgehog signaling and reducing neuroprotective factors, contributing to PD pathogenesis.
A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy
By onPublished: Structural and functional data support a model in which the TBK1-dependent phosphorylation of RAB7A serves as a switch, promoting mitophagy by relieving Rubicon inhibition and favoring Pacer activation. View original preprint.
Adult-specific Reelin expression alters striatal neuronal organization: implications for neuropsychiatric disorders
By onReelin levels might modulate the numbers of striatal interneurons and the density of the nigrostriatal dopaminergic projections, suggesting that these changes may be involved in the protection of Reelin against neuropsychiatric disorders.
EXPRESSION AND PURIFICATION OF HUMAN NEMO (GST-GFP-NEMO)
By onThis protocol describes how to express and purify human NEMO (IKK-γ) tagged N-terminally with GST and EGFP.
A data-driven single-cell and spatial transcriptomic map of the human prefrontal cortex
By onA data-driven molecular map of the DLPFC reveals distinct spatial domains and cell populations, offering insights into neuropsychiatric disorders. The study provides a roadmap for implementing spatial clustering approaches in the human brain.
Integrated multi-cohort analysis of the Parkinson’s disease gut metagenome
By onThe authors perform metagenomic sequencing of multiple geographically-disparate cohorts and find that stereotypic changes in the functional metabolic potential of the gut microbiome are a consistent feature of PD.
A prebiotic diet modulates microglial states and motor deficits in α-synuclein overexpressing mice
By onWhat should Parkinson's Disease patients eat? This study shows that dietary fiber impacts gut microbes and immune cells in the brain of a mouse model of Parkinson's.
Evaluation Of The Rims2 Locus As A Risk Locus For Parkinson’s Disease Dementia
By onLiu et al. found RIMS2 locus linked to dementia in Parkinson's disease. Our study with 2536 individuals found no association with RIMS2 or other loci. More research is needed to uncover biological factors influencing Parkinson's dementia.
CRISPR tagging of the TMEM192 gene with 3xHA in H9 ES AAVS-NGN2 cells for Lyso-IP
By onProtocol for adding a 3X HA tag to TMEM192 gene in human cells using CAS9 and recombination template is described for H9 ES AAVS1-NGN2 cells.
