Motor cortical neuronal hyperexcitability associated with α-Synuclein aggregation
By Emma Sherrell onThe results documented motor cortical neuronal hyperexcitability associated with αSyn aggregation and provided a novel mechanistic understanding of cortical circuit dysfunction in PD.
Poly-ornithine/laminin substrate for neural cell culture V.2
By Emma Sherrell onThis protocol is used to create adhesive and bioactive substrate for neural cell types, low to high density neurons, astrocytes, or organoids. It is based on standard methods but includes several optimizations, use case recommendations, alternatives, and advice.
Distributed dopaminergic signaling in the basal ganglia and its relationship to motor disability in Parkinson’s disease
By Emma Sherrell onDegeneration of dopaminergic neurons in the brain causes PD motor symptoms. Recent research shows dopamine's role in basal ganglia regions beyond striatum impact movement control. Restoring dopamine signaling outside the striatum can alleviate PD.
Primary data associated with the manuscript, “Loss of primary cilia and dopaminergic neuroprotection in pathogenic LRRK2-driven and idiopathic Parkinson’s disease” (doi: 10.1073/pnas.2402206121)
By Emma Sherrell onPrimary data (RNA sequencing; microscopy & imaging) associated with the manuscript, "Loss of primary cilia and dopaminergic neuroprotection in pathogenic LRRK2-driven and idiopathic Parkinson’s disease" (doi: 10.1073/pnas.2402206121).
Software for data analysis used in the manuscript, “Loss of primary cilia and dopaminergic neuroprotection in pathogenic LRRK2-driven and idiopathic Parkinson’s disease” (doi: 10.1073/pnas.2402206121)
By Emma Sherrell onSeurat version 3 was used for single-cell analysis, applying quality control steps to remove outliers and potential multiplets. Scripts and data are available on Dryad (https://doi.org/10.5061/dryad.pk0p2ngvp).
Source data – Cortical PFFs
By Emma Sherrell onSource data - Cortical PFFs for motor cortical neuronal hyperexcitability associated with α-Synuclein aggregation.
Gut-PFF surgery
By Emma Sherrell onMethod for injection of alpha-synuclein preformed fibrils in the gut in a mice.
Movement-related increases in subthalamic activity optimize locomotion
By Emma Sherrell onThe authors found most neurons in the STN exhibit increased activity during locomotion. Furthermore, optogenetic inhibition of this activity rapidly dysregulated gait. Thus, the STN facilitates movement and may drive locomotor activity in at-risk DA neurons.
hPSC passaging and propagation on laminin521 V.1
By Emma Sherrell onhPSC Passaging and propagation using Laminin521 and EDTA. Laminin actively supports survival, prevents spontaneous differentiation, and increases plating efficiency, thus net expansion, and enables 100% confluent single cell layer epithelial monolayer culture. EDTA-based (Gentle Cell Dissociation Reagent) minimizes stress and enhances viability. Cell lines require little or no adaptation.
Immunostaining – Fluorescent
By Emma Sherrell onStaining of mouse brain sections with immunofluorescent visualization.
Imaging – Confocal
By Emma Sherrell onProtocol for imaging using confocal microscope. Sections for analysis should be mounted on slides, stained for appropriate markers, and coverslipped. This protocol uses a Carl Zeiss LSM 880 confocal microscope.
Imaging – Bright field
By Emma Sherrell onProtocol for imaging using Hamamatsu NanoZoomer slide scanner. Sections for analysis should be mounted on slides, stained for appropriate markers, and coverslipped. This protocol uses a Carl Zeiss LSM 880 confocal microscope.
Cell counting
By Emma Sherrell onProtocol for counting cells labeled with immunofluorescent markers in mouse brain sections. Sections should be stained, mounted, and imaged with high resolution (2048 x 2048 scanning).
DNA extraction protocol for saliva samples obtained from Omigene oral OM device using the Qiagen QiaAMP Powerfecal DNA kit for shotgun metagenomic sequencing
By savannah onThis protocol describes the procedure for DNA extraction of saliva samples using the Omnigene OM device and the Qiagen QiaMP Powerfecal DNA PRO kit for shotgun metagenomic sequencing.
Data and images for Tonic dendritic GABA release by substantia nigra dopaminergic neurons
By savannah onDatasets from Simmons et al 2024.
Immunohistochemistry for brain sections
By Emma Sherrell onProtocol for processing 30um mouse brain sections for immunolabeling.
Nova-ST Spatial Transcriptomics protocol
By Emma Sherrell onNova-ST is an open-source, high-resolution sequencing-based spatial transcriptomics workflow. This method gives comparable resolution to BGI Stereoseq, SeqScope & PIXEL seq. Nova-ST is derived from dense nano-patterned randomly barcoded Illumina NovaSeq 6000 S4 sequencing flow cells.
Nuclei isolation, immunostaining, and Fluorescence-activated nuclei sorting (FANS) for Smart-Seq2
By Emma Sherrell onThis protocol describes the steps for extracting nuclei from human postmortem brain samples, immunofluorescence, and nuclei sorting (FANS) for single-nucleus RNA-seq using the Smart-Seq2 method.
