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EXPRESSION AND PURIFICATION OF HUMAN NEMO (GST-GFP-NEMO)
Output Details
Description
This protocol describes how to express and purify human NEMO (IKK-γ) tagged N-terminally with GST and EGFP. The expression is performed with E. coli Rosetta pLysS cells. The protein is purified via a GST batch purification and gel filtration (SEC).
Identifier (DOI)
10.17504/protocols.io.261gedpojv47/v1