Gene expression protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onGene expression protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
pLenti HsATP13A2 WT (PAM) _ Hygro
By Emma Sherrell onTransfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT with hygromycin selection marker.
Experimental design protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onExperimental design protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Ex vivo culture of SPMs protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onEx vivo culture of SPMs protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
pLenti HsATP13A2 P652_E653 del
By Emma Sherrell onTransfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del with puromycin selection marker.
Animal housing and ethical concerns protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onAnimal housing and ethical concerns protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Dautan et al. 2024, “Gut-Initiated Alpha Synuclein Fibrils Drive Parkinson’s Disease Phenotypes: Temporal Mapping of non-Motor Symptoms and REM Sleep Behavior Disorder”
By Emma Sherrell onThis zenodo record contains the raw data for the preprint including raw data csv files, images, matlab codes, imageJ macros, and Prism analysis files. See the README files for more details.
Rotarod test protocol used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onRotarod test protocol used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Genetically encoded and modular SubCellular organelle probes (GEM SCOPe) reveal lysosomal and mitochondrial dysfunction driven by PRKN
By Emma Sherrell onLysosomal and mitochondrial dysfunction are implicated in many diseases. GEM-SCOPe, a modular toolbox of fluorescent markers, helps visualize these organelles. In a PRKN-knockout model of PD, GEM-SCOPe identified disease-associated changes.
IHC and image analysis related to “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onIHC and image analysis related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Raw data of “The lipid flippase ATP10B enables cellular lipid uptake under stress conditions”
By Emma Sherrell onRaw data Manuscript Wouters et al BBA 2023.
Tissue and blood collection related to “LRRK2-G2019S Synergizes with Aging and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onTissue and blood collection related to "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Maintenance & differentiation: SHSY-5Y neuroblastoma cells
By Emma Sherrell onThis protocol details the maintenance and differentiation of SHSY-5Y neuroblastoma cells.
Western blot analysis V.1 used in “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onWestern blot analysis V.1 used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Primary glia isolation and culture protocol
By Emma Sherrell onThis protocol details the isolation and maintenance of microglia, oligodendrocytes, and astrocytes derived from early postnatal primary mouse cortex.
Magic red cathepsin B activity assay
By Emma Sherrell onThis protocol details the magic red cathepsin B activity assay for cultured cells.
Statistical analysis protocol for “LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration”
By savannah onStatistical analysis protocol for "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Lysosomal isolation protocol
By Emma Sherrell onThis protocol details the isolation of lysosomes from in vitro cultured cells.
List of IHC/WB antibodies, RT-PCR probes, and software used
By savannah onList of IHC/WB antibodies, RT-PCR probes, and software used in "LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration."
Lysosomal membrane permeability (LMP) assay
By Emma Sherrell onThis protocol details the use of a lysosomal membrane permeability (LMP) assay following cell treatment and fixation.
