pAAVDJ-CAG-FlpX-mKate2.0-N2cG
By onThe pAAV-CAG-fDIO-mKate2.0-N2cG vector was used as a helper virus in conjunction with a rabies virus to label presynaptic inputs to various regions in the brain with focus on SNr neurons.
ATG2A engages Rab1a and ARFGAP1 positive membranes during autophagosome biogenesis
By onATG2A aids in autophagosome formation by localizing to extra-Golgi ARFGAP1 puncta with Rab1 during early stages. Rab1 is crucial for autophagy, and disruptions in autophagosome formation lead to accumulation of ARFGAP1 and Rab1a at ectopic sites.
Soluble Immune Factor Profiles in Blood and CSF Associated with LRRK2 Mutations and Parkinson’s Disease
By onThis preprint explores immune factors in Parkinson's disease linked to LRRK2 mutations. Serum showed elevated SDF-1 alpha and TNF-RII levels in LRRK2 carriers, while CSF had reduced immune markers. PD patients displayed reduced CSF inflammation.
Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons
By onThis study compared the efficacy and selectivity of 11 different AAV serotypes for the efficient and selective transduction of murine olfactory sensory neurons in vivo. The findings inform research approaches and gene therapy based initiatives.
Advances in mitophagy initiation mechanisms
By onThis review will cover recent advances in our understanding of how mitophagy is initiated, and by doing so reveal the mechanistic plasticity of how autophagosome formation can begin.
Stereotaxic Surgery
By onThis protocol describes the steps for performing mouse stereotaxic surgery. It is applicable to intracranial injections of viruses and drugs.
Postsynaptic adaptations in direct pathway muscarinic M4-receptor signaling follow the temporal and regional pattern of dopaminergic degeneration
By onImbalances in dorsal striatum output in Parkinson's disease are driven by dopamine loss and disrupted acetylcholine signaling. These changes occur in response to dopamine loss, affecting M4 receptors in striatal neurons, crucial for PD progression.
6-OHDA mouse model of Parkinson’s disease
By onProtocol for creating a Parkinson's disease mouse model using 6-OHDA involves injecting different doses into the brain to induce varying levels of dopaminergic damage.
Levodopa treatment for low-dose 6-OHDA-treated mice
By onThis protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Electrophysiology and 2-photon imaging of Ca+2-transients
By onProtocol for imaging dendritic calcium transients using 2-photon microscopy with a Ti:Sapphire laser. Fluorescence collected through objective, dichroic mirror, and filters, then detected by a GaAsP PMT. Output digitized for analysis.
Ex Vivo Electrophysiology – Ford Lab
By onThis protocol describes the steps to perform whole-cell electrophysiology recordings in acute brain slices.
Motor Behavioral Assessment
By onProtocol outlines motor behavior tests for Parkinson's disease in mice using open field, rotarod, cylinder, and balance beam tests. Mice were habituated for 30-40 min before tests conducted 3-4 weeks post-injections.
Mouse Whole Gut Transit Time
By onProtocol used in the Gradinaru lab for obtaining whole gut transit time for mice
Current safety recommendations for handling mouse and human αsynuclein pre-formed fibrils
By onThe paper outlines best practices in conducting research with α-synuclein fibrils. We highlight steps in which extra precautions should be taken and how to minimize exposure and potential risk associated with use of PFFs in scientific research.
Coronal cryosectioning of mouse brains
By onProtocol used in the Gradinaru lab for coronal cryosectioning of mouse brain tissue with a cryostat
Immunostaining Mouse Tissue
By onProtocol used in the Gradinaru lab for immunohistochemistry to analyze protein expression in the mouse brain
RNA extraction from organoids
By onProtocol used in the Gradinaru lab for RNA extraction from organoids
