Virus production and administration
By Julia Julia Leonard onProtocol for AAV production and administration to mice.
HEK293E_Clu CC3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
Tissue preparation, immunohistochemistry, imaging, and quantification
By Julia Julia Leonard onProtocol for neuronal imaging.
HEK293E_Clu CC2
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
GCaMP6f fluorescence in ex vivo intestinal preparation
By Julia Julia Leonard onProtocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.
HEK293E_Clu CC1
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu AB2
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
Metagenome preparation and analysis
By Julia Julia Leonard onProtocol for metagenomic analysis from mouse fecal pellets.
Expansion microscopy with R1441C LRRK2 MEF cells: Visualization of Myc-RILPL1 and TMEM55B
By Emma Sherrell onExpansion microscopy is a super-resolution imaging technique that uses expandable hydrogels to increase the physical distance between fluorophores from fixed cells on coverslips. By isotropically expanding samples, the traditional wavelength-limited confocal microscopy imaging techniques can be improved upon ~4X in resolution. Here, the authors use expansion techniques developed previously by others to confocal image TMEM55B and RILPL1.
Determination of NM concentration
By Emma Sherrell onThis is the protocol for determining neuromelanin concentration and data.
Metabolomics preparation and analysis
By Julia Julia Leonard onProtocol for metabolomic analysis from mouse fecal and cecal samples.
Chemical degradation and determination of pheomelanin and eumelanin markers
By Emma Sherrell onThis is a protocol involving chemical oxidation and reduction methods followed by high-performance liquid chromatography (HPLC) detection of markers for pheomelanin and eumelanin.
Proteome preparation and analysis
By Julia Julia Leonard onProtocol for proteomic analysis from mouse cecal samples.
3′ mRNA-sequencing
By Julia Julia Leonard onProtocol for 3' mRNA sequencing of mouse gastrointestinal tract tissues.
Whole gut transit time, fecal water content, and fecal output
By Julia Julia Leonard onProtocol for mouse gastrointestinal function assays (whole gut transit time, fecal water content, and fecal output).
Characterization of splay characteristics of carbon fiber electrode threads (CFETs) in agar brain phantom
By Emma Sherrell onMethods to measure deflection characteristics of carbon fiber electrodes in agar brain phantoms.
Colonic migrating motor complexes
By Julia Julia Leonard onProtocol for ex vivo assay of colonic migrating motor complexes (CMMCs) in mouse colons.
Central and peripheral innate and adaptive immunity in Parkinson’s disease
By Emma Sherrell onParkinson’s disease (PD) is a multisystem disorder with characteristics of a chronic inflammatory disease. To develop effective immunomodulatory interventions to combat PD, researchers need to think innovatively about the implications of orchestrated central and peripheral innate and adaptive immune responses that occur as the disease begins and progresses.
CD8 cell density In substantia nigra and cerebral peduncle image analysis
By Emma Sherrell onQuPath is a bioimage analysis software designed for digital pathology and whole slide image analysis. This protocol describes how to measure CD8 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Pathways controlling neurotoxicity and proteostasis in mitochondrial complex I deficiency
By Julia Julia Leonard onNeuromuscular disorders caused by dysfunction of the mitochondrial respiratory chain are common, severe, and untreatable. The authors recovered a number of mitochondrial genes, including electron transport chain components, in a large forward genetic screen for mutations causing age-related neurodegeneration in the context of proteostasis dysfunction.