mtHsp60:mtHsp10 football complex, cryo-ET STA from HeLa
By onSubtomogram average of the mtHsp60:mtHsp10 football complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
In situ cryo-ET dataset of mitochondria in HeLa cells
By onDataset of tilt-series from in situ cry-electron tomography. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
mtHsp60 single- ring complex, cryo-ET STA from HeLa
By onSubtomogram average of the mtHsp60 single- ring complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
mtHsp60:mtHsp10 half-football complex, cryo-ET STA from HeLa
By onSubtomogram average of the mtHsp60:mtHsp10 half-football complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
55S mitochondrial ribosome, cryo-ET STA from HeLa
By onSubtomogram average of the 55S mitochondrial ribosome, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
39S mitochondrial ribosome, cryo-ET STA from HeLa
By onSubtomogram average of the 39S mitochondrial ribosome, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
A 4-5X expansion microscopy protocol for high‑resolution imaging of olfactory sensory neuron cilia in mouse olfactory epithelium
By onProtocol for expanding mouse olfactory epithelium for improved imaging: OE dissected on a cold platform, embedded in hydrogel, and expanded 4-5 times with ExM. Enables detailed visualization of ciliary structures without super-resolution microscopy.
Selective loss of Primary Cilia and Neurotrophic Signaling in G51D α-Synuclein Mice Highlights a Common Pathway to Parkinson’s Disease
By onG51D α-synuclein mice mimic disease symptoms, showing cilia loss in specific neurons and impaired neurotrophic signaling, contributing to disease progression. This highlights the role of ciliary dysfunction in Parkinson’s.
Cardiac-sympathetic state predicts action restraint, gated by demonstrated agency
By onIn healthy humans, beat-to-beat cardiac contractility during incentivized reaching predicts action restraint. Cardiac-sympathetic outflow flexibly shapes speed–accuracy tradeoffs under reward and loss contexts, beyond simple action mobilization.
A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy
By onPublished: Structural and functional data support a model in which the TBK1-dependent phosphorylation of RAB7A serves as a switch, promoting mitophagy by relieving Rubicon inhibition and favoring Pacer activation. View original preprint.
Adult-specific Reelin expression alters striatal neuronal organization: implications for neuropsychiatric disorders
By onReelin levels might modulate the numbers of striatal interneurons and the density of the nigrostriatal dopaminergic projections, suggesting that these changes may be involved in the protection of Reelin against neuropsychiatric disorders.
Integrated multi-cohort analysis of the Parkinson’s disease gut metagenome
By onThe authors perform metagenomic sequencing of multiple geographically-disparate cohorts and find that stereotypic changes in the functional metabolic potential of the gut microbiome are a consistent feature of PD.
EXPRESSION AND PURIFICATION OF HUMAN NEMO (GST-GFP-NEMO)
By onThis protocol describes how to express and purify human NEMO (IKK-γ) tagged N-terminally with GST and EGFP.
A data-driven single-cell and spatial transcriptomic map of the human prefrontal cortex
By onA data-driven molecular map of the DLPFC reveals distinct spatial domains and cell populations, offering insights into neuropsychiatric disorders. The study provides a roadmap for implementing spatial clustering approaches in the human brain.
A prebiotic diet modulates microglial states and motor deficits in α-synuclein overexpressing mice
By onWhat should Parkinson's Disease patients eat? This study shows that dietary fiber impacts gut microbes and immune cells in the brain of a mouse model of Parkinson's.
Characterizing spatial and temporal properties of ER-phagy receptors
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
