Optical densitometry of neuronal fibers
By Emma Sherrell onOptical densitometry of striatal neuronal fibers in the rodent brain.
Immunohistochemistry on free-floating cryosections
By Emma Sherrell onImmunochemistry protocol on rodent brain cryosections.
Hematoxylin and Eosin (H&E) staining
By Emma Sherrell onHematoxylin and Eosin (H&E) staining for histological sections.
Nissl counterstaining – Cresyl Violet
By Emma Sherrell onNissl Counterstaining with Cresyl Violet for histological sections.
Direct ELISA
By savannah onThis protocol details how to set up and run a direct ELISA on your protein of interest.
Miniscope calcium imaging data acquisition of cortical activity in non-human primates (NHPs)
By savannah onThe protocol describes head-mounted miniscope calcium imaging data acquisition for NHPs.
Primary data part 2 for synaptic tracing for article, “Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input” (DOI: 10.1016/j.celrep.2024.114483)
By savannah onStudy in FosTRAP mice examines striatal neurons linked to dyskinesia using various methods. Dataset relates to synaptic tracing in levodopa-induced dyskinesia. Published in Ryan et al., 2024 (Cell Reports).
Development and characterization of a non-human primate model of diseeminated synucleinopathy
By savannah onA viral vector was used in NHP to spread a mutated gene, inducing synucleinopathy in brain areas connected to the putamen. After four weeks, animals were studied to quantify affected neurons and their distribution.
Datasets, code, and key resources table from Brimblecombe, K. et al. (2023): “Inhibition of striatal dopamine release by the L-type calcium channel inhibitor isradipine co-varies with risk factors for Parkinson’s”
By savannah onThe following files are associated with Brimblecombe, K. et al. (2023) study: (a) Key Resources Table, (b) all tabular datasets, and (c) Custom R code used to analyze all datasets.
Purification of mCherry-ATG101/13 (1-191aa) subcomplex
By savannah onThis protocol describes how to express and purify mCherry-ATG101/13 (1-191aa) subcomplex.
Purification of mCherry- or GFP-tagged ATG13/101 subcomplex
By savannah onThis protocol describes how to express and purify mCherry- or GFP-tagged ATG13/101 subcomplex.
Purification of GST-WIPI1/WIPI2d/WIPI3/WIPI4
By savannah onThis protocol describes how to express and purify GST-WIPI1/WIPI2d/WIPI3/WIPI4.
Purification of mCh-WIPI2d-IDR (364-426aa)
By savannah onThis protocol describes how to express and purify mCh-WIPI2d-IDR (364-426aa).
Purification of mCherry-WIPI2d/WIPI3
By savannah onThis protocol describes how to express and purify mCherry-WIPI2d/WIPI3.
Purification of Lambda Protein Phosphatase
By savannah onThis protocol describes how to express and purify Lambda Protein Phosphatase.
Transgenic mouse colony maintenance
By Emma Sherrell onMethods for the generation and maintenance of transgenic tyrosinase mice.
Purification of CK2 kinase complex
By savannah onThis protocol describes how to express and purify CK2 kinase complex.
In situ hybridization for Tyrosinase
By Emma Sherrell onIn situ hybridization protocol with DIG-labelled riboprobes.
