Mito-IP: Immunopurification of Mitochondria from MitoTag Cultured Cells
By onA novel method called 'Mito-IP' enables rapid and pure mitochondrial isolation in 10 minutes, supporting various analyses. This technique is being extended to isolate other organelles like lysosomes, Golgi, and peroxisomes using epitope tags.
GCase assay – Resorufin β-D-glucopyranoside
By onMethod presented for assessing lysosomal enzyme GBA1 activity using Resorufin substrate in low pH conditions to minimize non-lysosomal activity. Validated in GBA1 KO cells, compatible with various lysates and adjustable for different GBA1 levels.
Mouse brain immunohistochemistry – colorimetric analysis
By onProtocol for colorimetric analysis of mouse brain immunohistochemistry is described.
A CellProfiler computational pipeline to quantify localization of PPM1H on mitochondria
By onCellProfiler software pipeline quantifies PPM1H localization on fragmented mitochondria by hypotonic swelling, highlighting membrane contacts. Wild-type MEFs expressing PPM1H-mApple and GFP-Mito are imaged after treatment with oligomycin/antimycin.
p-S65-Ub sandwich ELISA
By onAssay measures Ubiquitin phosphorylation at Serine-65 in vivo using ELISA. Method previously detailed by Watzlawik et al. in their publication.
PINK1 WT/LRRK2 WT, PINK1 WT/LRRK2 RC, PINK1 KO/LRRK2 WT, PINK1 KO/LRRK2 RC
By onMice with LRRK2 R1441C and PINK1 KO mutations were crossed to create double mutant lines: LRRK2 WT/PINK1 WT, LRRK2 RC/PINK1 WT, LRRK2 WT/PINK1 KO, and LRRK2 RC/PINK1 KO.
LRRK2 R1441C/PINK1 Double mutant mouse embryonic fibroblasts
By onLRRK2 R1441C/PINK1 double mutant mouse embryonic fibroblasts were studied alongside homozygous mutant and littermatched wild-type cells.
Primary data1: Pathogenic LRRK2 mutations cause loss of primary cilia and Neurturin in striatal parvalbumin interneurons
By onPathogenic LRRK2 mutations lead to loss of primary cilia and Neurturin in striatal parvalbumin interneurons, as indicated in the primary data for the manuscript.
Primary data2: Pathogenic LRRK2 mutations cause loss of primary cilia and Neurturin in striatal parvalbumin interneurons
By on*LRRK2* mutations lead to loss of primary cilia and Neurturin in striatal parvalbumin interneurons, as found in the manuscript.
Isolation of brain eGFP-cholinergic interneurons by dissociation and flow cytometry
By onProtocol isolates GFP-expressing cholinergic interneurons from mouse dorsal striatum tissue using a method by Lynette Foo (2013).
Part1 Raw data for: Lysosomal glucocerebrosidase is needed for ciliary Hedgehog signaling: A convergent pathway contributing to Parkinson’s disease
By onRaw data for Figures 1-5 in a study on the role of lysosomal glucocerebrosidase in ciliary Hedgehog signaling, linking to Parkinson's disease.
Part2 Raw data for: Lysosomal glucocerebrosidase is needed for ciliary Hedgehog signaling: A convergent pathway contributing to Parkinson’s disease
By onThe raw data for Figure 6 in the manuscript supports the role of lysosomal glucocerebrosidase in ciliary Hedgehog signaling, linking it to Parkinson's disease.
Data for: Allosteric regulation of the Golgi-localized PPM1H phosphatse by Rab GTPases modulates LRRK2 substrate dephosphorylation in Parkinson’s disease LRRK2-counteracting PPM1H phosphatase
By onRaw tabular data and original TIFF files available for Western blots. Raw tabular data provided for all MST binding experiments.
Lrrk2 flox-R1441G
By onDardarin/Lrrk2 regulates inflammation, protein processing, and autophagy. Mutations, like R1441G, linked to Parkinson's disease. LRRK2 flox-R1441G mice model the disease for research, showing normal phenotype until gene manipulation.
ASAP Research Round-Up | Q3 2025
In this second edition of the ASAP Research Round-Up, ASAP shares advancements in Q2 2025 across the ASAP portfolio that fill critical knowledge gaps, promote rapid dissemination of scientific insights, expand resource accessibility, and support the next generation of Parkinson’s researchers.
Gene expression profile at single cell levels of the cells from the controls and tau P251L Drosophila brain
By onTau protein's role in Alzheimer's disease was studied by introducing a human tau mutation into Drosophila using CRISPR. Single-cell RNA sequencing helped identify genes and pathways involved in tauopathy.
