CXCL1 supplementation on iPSC-Derived Midbrain Neurons
By onThe protocol studies the impact of different levels of recombinant human CXCL1 on midbrain neurons from iPSCs using conditioned media from ATP13A2WT astrocytes.
Bulk RNA-seq Protocol for Astrocytes
By onProtocol for RNA extraction from astrocytes for bulk RNA sequencing to analyze gene expression profiles.
SAM ELISA
By onProtocol for preparing cell lysates and conducting ELISA to quantify proteins is outlined.
Fluorescently labeled polyamine uptake (via confocal microscopy)
By onMethod visualizes polyamine uptake in cells with fluorescently labeled polyamines & confocal microscopy.
CXCL1 ELISA
By onProtocol for ELISA includes sample prep & assay to quantify antigens in biological samples.
Fluorescently labeled polyamine uptake (via flow cytometry)
By onSupplemental video in ASAP Protocol Particulars series provides extra context and tips from protocol authors for Aligning Science Across Parkinson's project.
Differentiation of iPSCs into NGN2 Neurons Protocol
By onProtocol uses doxycycline to induce neurogenin 2 in iPSCs for neuron differentiation, aiding research on neuronal development and function.
Human Induced Pluripotent Stem Cell (hiPSC) Culture and Passaging Protocol
By onProtocol for hiPSC maintenance and passage using Geltrex-coated plates and StemFlex medium for optimal growth and reduced differentiation.
Differentiation of Human Induced Pluripotent Stem Cells into Midbrain Neurons
By onProtocol uses specific media and growth factors to convert hiPSCs into midbrain neurons with high purity and reproducibility for transplantation and disease modeling.
Protocol for Dissociation and Culture of Midbrain Astrocytes and Neurons
By onProtocol for isolating and culturing midbrain astrocytes and neurons in specific ratios to preserve viability and differentiation potential for future research purposes.
Astrocyte-Conditioned Media (ACM) cultures
By onAstrocyte-conditioned media (ACM) is created for research on D25 midbrain organoids and 2D neuronal cultures.
Protocol for Live-Cell Imaging of Astrocytes Exposed to HiLyte™ 488-labeled Human Recombinant α-Synucleins
By onProtocol studies impact of HiLyte™ 488-labeled human recombinant α-synuclein on astrocytes through live-cell imaging at different time points post-exposure.
Quantification of Cell Death and Cytotoxicity Using LDH-Glo™ Cytotoxicity Assay
By onThe LDH-Glo™ Cytotoxicity Assay quantifies cell death and cytotoxicity in cultured cells by measuring LDH released from damaged cells.
Evaluation of the Inflammatory Secretome of Astrocytes Using Cytokine Array
By onAstrocyte secretome studied with Proteome Profiler™ Human XL Cytokine Array Kit to analyze and quantify cytokine expression levels in conditioned media.
ASAP Research Round-Up | Q1 2026
In this edition of the ASAP Research Round-Up, ASAP presents Q1 2026 highlights from across its portfolio, showcasing efforts to advance Parkinson’s discovery, facilitate the rapid global exchange of ideas, broaden open-science accessibility, and support the next generation of Parkinson’s researchers.
Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
By onThis methods paper outlines silencing transposable elements in hiPSCs using CRISPRi. Describes gRNA design, validation via multiome approach. Enables functional studies on TE transcription in hiPSC models.
Behavioral screening defines the molecular Parkinsonism-related subgroups in Drosophila
By onWe created a new collection of 24 genetically well-controlled Drosophila models of familial forms of parkinsonism. Using unbiased behavioral screening and machine learning we identified three clusters of mutants that converge.
PD5D long read DNA-seq
By onThe dataset includes CCS corrected HiFi lrDNAseq in FASTQ format for 100 PMDBS samples from Parkinson's patients and controls. Part of PD5D atlas, subjects were also profiled with genotyping, single-cell ATACseq, and spatial transcriptomics.
