Evaluation Of The Rims2 Locus As A Risk Locus For Parkinson’s Disease Dementia
By onLiu et al. found RIMS2 locus linked to dementia in Parkinson's disease. Our study with 2536 individuals found no association with RIMS2 or other loci. More research is needed to uncover biological factors influencing Parkinson's dementia.
CRISPR tagging of the TMEM192 gene with 3xHA in H9 ES AAVS-NGN2 cells for Lyso-IP
By onProtocol for adding a 3X HA tag to TMEM192 gene in human cells using CAS9 and recombination template is described for H9 ES AAVS1-NGN2 cells.
Analysis of ER Flux in Cultured Induced Neurons using Keima ER reporters
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Characterizing spatial and temporal properties of ER-phagy receptors
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Analysis of ER structures in Cultured Induced Neuron axons
By onAssociated with preprint: https://doi.org/10.1101/2023.06.26.546565
Microscopy-based bead assay
By onThis protocol describes the procedure to perform microscopy-based bead assay.
DAB ANTIBODY (IHC) STAINING PROTOCOL
By onThis is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.
SNP Genotyping and ApoE Genotyping
By onProtocol outlines DNA extraction from blood, quality control, SNP, and APOE genotyping. Adapted from PRoBaND SNP Genotyping and ApoE Genotyping Protocol by Malek et al. for Parkinson's Disease study.
CoxII degradation assay to assess mitophagy
By onThis protocol details the procedure to assess mitophagy by analyzing COXII degradation via Western blotting.
Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
By onThis protocol describes the maintenance and expansion of iPSCs in the adherent culture via single-cell passaging.
Primary data associated with Figure 3–Figure Supplement 4 in doi: 10.1101/2022.04.25.489459
By onRaw immunoblotting data and tabular data for quantitation used to generate Figure 3–Figure Supplement 4 in doi: 10.1101/2022.04.25.489459 ("A Feed-forward Pathway Drives LRRK2 kinase Membrane Recruitment and Activation").
R Code used in “Sex-Specific Microglial Responses to Glucocerebrosidase Inhibition: Relevance to GBA1-Linked Parkinson’s Disease”
By onR Code used in "sex-specific microglial responses to glucocerebrosidase inhibition: Relevance to GBA1-linked Parkinson’s disease."
Protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By onStep by step procedure for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice.
Feeder-free culturing of hPSCs
By onThis collection describes the standard procedure of feeder-free culturing of human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex
Coating plates
By onThis protocol describes the process of coating plates using either VTN, Matrigel or Geltrex for use in culturing of feeder-free human pluripotent stem cells (hPSCs)
Standard Operating Procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates
By onThis protocol details standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates.
