Polarization camera PAINT movie of single Nile red dyes binding to lipid bilayer-coated silica microspheres
By savannah onPolarization camera PAINT movie of single Nile red dyes binding to lipid bilayer-coated silica microspheres.
Polarization camera dSTORM datasets of actin in fixed HeLa cells labeled with phalloidin-Alexa Fluor 488
By savannah onPolarization camera dSTORM dataset of the actin of fixed HeLa cells labeled with phalloidin-Alexa Fluor 488.
Polarization camera dSTORM datasets of actin in fixed HeLa cells labeled with phalloidin-Alexa Fluor 647
By savannah onPolarization camera dSTORM dataset of the actin of fixed HeLa cells labeled with phalloidin-Alexa Fluor 647.
Polarization camera movies of T cells on a cover glass
By savannah onPolarization camera movies of T cells on a cover glass.
Human post-mortem nuclei preparation and single-nucleus multiome DNA/RNA sequencing by ResolveOME
By savannah onHuman post-mortem nuclei preparation and single-nucleus multiome DNA/RNA sequencing by ResolveOME.
Video S1 SYTOX Orange
By savannah onSupporting movie showing single SYTOX Orange molecules dispersed on a cover glass.
The role of alpha-synuclein pathology
By Emma Sherrell onThis book chapter will be published (open-access) in October 2024. Book title: Translational Methods for Parkinson’s Disease and Atypical Parkinsonism Research. Editors: Sergiu Groppa, Susanne Schneider.
Rotarod test
By Emma Sherrell onBehavioral assay to measure motor impairments, specifically balance and coordination, in mice.
Polarization camera movie of single SYTOX Orange molecules on a cover glass
By savannah onRoSE-O adapted for SMOLM using polarization camera. Can also be modified to work with other single-channel imaging systems.
Cognitive dysfunction in animal models of human lewy-body dementia
By Emma Sherrell onSynucleinopathies like Parkinson’s disease dementia are linked to cognitive impairments due to Lewy bodies. Various methods, like genetic manipulation and toxin exposure, can model these pathologies in research to understand human diseases better.
RoSE-O_POLCAM
By savannah onThis repository contains MATLAB scripts and utilities for the analysis of simulated and experimental POLCAM single-molecule orientation-localisation microscopy (SMOLM) data using RoSE-O, providing accurate and precise measurements of the location and orientation of single molecules.
6-OHDA lesion in medial forebrain bundle
By Emma Sherrell onLesion of the medial forebrain bundle using 6-OHDA to induce Parkinsonian-like deficits in mice.
AAV craniotomy
By Emma Sherrell onThis protocol is for the injection of AAV into a mouse brain using a stereotactic frame and Hamilton syringe.
POLCAM-SR: Version 1.4
By savannah onSoftware for processing single-molecule and diffraction limited polarization camera image data.
Apomorphine-induced rotations
By Emma Sherrell onTo test for 6-OHDA lesion efficiency, rotational behaviors are assessed while mice are under the effects of apomorphine. This test is run during the dark phase of light cycle. Experiments are performed by an examiner blinded to treatment groups.
