Constant potential amperometry in vitro
By Julia Julia Leonard onProtocol for constant potential amperometry recordings in vitro of evoked dopamine release in the rat striatum.
Rotarod test
By Julia Julia Leonard onProtocol to assess motor coordination and balance by using an accelerating rod.
Open field test
By Julia Julia Leonard onThe open field test is a simple test to evaluate general motor and explorative behavior in rodents.
pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA
By Julia Julia Leonard onA viral construct designed to express GCaMP6m pan-neuronally and express a red fluorophore (mRuby) in the nucleus of cortical interneurons. This construct is ideal for dual-imaging experiments to capture activity of two neuronal populations and to identify interneurons with red fluorescence in parallel or post-mortem.
pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack
By Julia Julia Leonard onBright green (mNeon) and red (mRuby) fluorescence proteins are expressed, with the former being cytoplasmic and the latter membrane-targeted. This expression is ideal for tracking long diffuse axons in cortical structures in vivo or for slice histology and cleared brain tissues post-mortem.
pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE
By Julia Julia Leonard onThis links GCaMP8f with a ribosome-tethered nanobody that restricts GCaMP to the soma of neurons. Particularly useful in very dense nuclei with strong neuropil, which can make post-hoc cell segmentation (i.e., independent component analysis (ICA) or constrained nonnegative matrix factorization (CMNF)) difficult.
pAAV_Ef1a_DIO_hTyrNull_HATag
By Julia Julia Leonard onCre-dependent expression of a truncated human tyrosinase is inactive and does not lead to the accumulation of neuromelanin in catecholaminergic neurons (control virus). Strong expression of Ef1a promoter, HA tag for histological verification of expression.
pAAV_Ef1a_DIO_hTyrHA_minBack
By Julia Julia Leonard onCre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. Expression under strong Ef1a promoter.
pAAV_Cbh_DIO_hTyr
By Julia Julia Leonard onCre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. The CbH promoter is a strong, pan-neuronal, non-silencable promoter.
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
By Julia Julia Leonard onThis construct links GCaMP8s to Synaptophysin and directs the fusion protein to presynaptic sites. It also produces a cytoplasmic red fluorescence protein (mRuby) via the self-splicing peptide 2A. It is mainly used to longitudinally image synaptic activity with 2-Photon at 980nm and visualize axons at 1080nm in vivo.
Induction of aggregation in alpha-synuclein-expressing cells by treatment with preformed fibrils (PFFs)
By Julia Julia Leonard onThis protocol details how to efficiently produce alpha-synuclein aggregates in cells by templating the misfolding of intracellular alpha-synuclein through treatment with preformed fibrils of alpha-synuclein (PFFs).
Kufor-Rakeb Syndrome-Associated Psychosis: A novel loss-of-function ATP13A2 variant and response to treatment
By Julia Julia Leonard onBiallelic (autosomal recessive) pathogenic variants in ATP13A2 cause a form of juvenile-onset parkinsonism, termed Kufor-Rakeb syndrome. The article describes the treatment response in an individual with Kufor-Rakeb syndrome-associated psychosis.
Source data for “Feed-forward metabotropic signaling by Cav1 Ca2+ channels supports pacemaking in pedunculopontine cholinergic neurons”
By Julia Julia Leonard onSource data and images from the paper: Feed-forward metabotropic signaling by Cav1 Ca2+ channels supports pacemaking in pedunculopontine cholinergic neurons.
Quantification of area and optical density of intracellular neuromelanin with Image J
By Julia Julia Leonard onQuantification of area and optical density of intracellular neuromelanin with Image J.
Protocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections
By Julia Julia Leonard onProtocol for preparing post-mortem tissue for intracellular neuromelanin quantification in H&E-stained sections.
Quantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections
By Julia Julia Leonard onQuantification of area and optical density of intracellular neuromelanin with TruAI in H&E stained sections.
Toward a standard model for autophagosome biogenesis
By Emma Sherrell onTwo papers in this issue resolve a long-standing obstacle to a “standard model” for autophagosome biogenesis in mammals.
The annotation and function of the Parkinson’s and Gaucher disease-linked gene GBA1 has been concealed by its protein-coding pseudogene GBAP1
By Emma Sherrell onHere, the authors identify novel transcripts from both GBA1 and GBAP1, including protein-coding transcripts that are translated in vitro and detected in proteomic data, but that lack GCase activity.
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease: Bioinformatic prioritization and hit validation
By Emma Sherrell onThis protocol describes the Bioinformatic Prioritization of PD GWAS candidates for High Content Screening, and Hit Validation by allele-specific expression (ASE) analysis.
Primary data associated with the manuscript, “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (3/3)
By Emma Sherrell onPrimary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase."