Synapse staining – mouse brain sections
By onThis protocol details a method to visualize and quantify excitatory synapses using mouse brain sections.
Isolation and culture of primary mouse astrocytes and neurons
By onThis protocol details the methodology to establish primary murine astrocyte and neuron cultures.
Brain slice preparation for electrophysiology recording
By onThis protocol details the preparation of acute brain slices from 8-week-old mice for whole-cell patch-clamp electrophysiology.
pYSG-IBA162-FLAG-hATP13A4-TwinStrep
By onExpression of human Atp13A4 (FLAG-tag, TwinStrep-tag) in yeast
pGAE-SFFV-BsdR-1x miR4_MmATP13A4_WPRE
By ontransfer plasmid for lentiviral vector production with miR for Mm ATP13A4
pGAE-SFFV-BsdR-1x miRFLUC_MmATP13A4_WPRE
By ontransfer plasmid for lentiviral vector production with miR for firefly luciferase (FLUC)
pGAE-SFFV-BsdR-1x miR2_MmATP13A4_WPRE
By ontransfer plasmid for lentiviral vector production with miR for Mm ATP13A4
pGAE-SFFV-BsdR-1x miR3_MmATP13A4_WPRE
By ontransfer plasmid for lentiviral vector production with miR for Mm ATP13A4
pCHMWS-hATP13A4_WT-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 WT
pCHMWS-hATP13A4_WT-Twinstrep-Flag-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 WT with Twinstrep-Flag tag
pCHMWS-hATP13A4_E646D-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 E646D mutant
pCHMWS-hATP13A4_E646D-Twinstrep-Flag-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 E646D mutant with Twinstrep-Flag tag
pCHMWS-hATP13A4_D486N-Twinstrep-Flag-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 D486N mutant with Twinstrep-Flag tag
pCHMWS-hATP13A4_E276K-Twinstrep-Flag-IRES-Puro
By ontransfer plasmid for lentiviral vector production expressing Hs ATP13A4 E276K mutant with Twinstrep-Flag tag
Para-subthalamic nucleus adjoins subthalamic nucleus and medial forebrain bundle, major DBS-targets in Parkinsons disease, OCD, and depression
By onThe para-STN is a poorly-defined region near critical DBS targets for PD. Here, we construct anatomical maps of para-STN and STN in multiple species using novel molecular markers in conjunction with FISH and light-sheet microscopy.
Scientists to Study Link Between Pesticides and Parkinson’s
Parkinson’s News Today reports that the ASAP has awarded a $9 million grant to scientists from Cedars-Sinai, to investigate how exposure to specific pesticides and air pollutants interacts with genetic vulnerabilities to trigger the development of the disease.
