Rodent brain processing for histological analyses
By Emma Sherrell onProtocol for rat brain processing in order to perform histological analyses.
Imaging single SYTOX Orange molecules on a PLL-coated cover glass V.1
By savannah onProtocol for preparing a microscopy sample of single SYTOX Orange molecules on PLL-coated cover glass, used in Bruggeman et al.'s POLCAM study for molecular orientation microscopy. Published in bioRxiv, Feb 2023.
Preparation and imaging of lipid bilayer-coated silica microspheres
By savannah onProtocol details lipid bilayer-coated glass beads imaging for molecular orientation microscopy.
TAB-PAINT imaging of alpha-synuclein fibrils using Nile Red
By savannah onProtocol for preparing alpha-synuclein fibrils on PLL-coated cover glass for TAB-PAINT imaging with Nile Red.
dSTORM of actin in fixed HeLa cells
By savannah onProtocol for preparing HeLa DTS cells for dSTORM imaging of actin network.
Live-cell imaging of the plasma membrane of Jurkat T cells
By savannah onProtocol for preparing Jurkat T cells for live imaging of plasma membrane, used in Bruggeman et al.'s study on POLCAM microscopy. Data in Figure 6 of bioRxiv publication.
AAV production and purification
By savannah onThis protocol is for the production and purification of Adeno-Associated Virus. The protocol contains the necessary steps to produce AAVs from HEK293T cell cultures.
DAB staining for Tyrosine Hydroxylase (TH) on free-floating fixed NHP brain tissue
By savannah onThis protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining for Tyrosine Hydroxylase (TH) of free-floating fixed brain tissue sections using the avidin/biotin ABC complex and the Rabbit Polyclonal Anti-TH NB300-109 (Novus).
Chronic multi-electrode assembly implantation with a chamber setup
By savannah onProtocol for implanting carbon fiber microelectrodes for chronic recording using a two-part chamber system in rhesus macaques. Prior steps for chamber implantation are detailed in other protocols.
Craniotomy, second, and third phase implantation to complete two-part chamber system
By savannah onThis protocol describes how to install the second or third phase of the two-part chamber system in rhesus macaques, as well as the craniotomy. The first baseplate installation is described in a separate protocol titled, "Baseplate Implantation for Two-Part Chamber System in Rhesus Monkeys."
Baseplate implantation for two-part chamber system
By savannah onProtocol details implanting a baseplate onto the skull as the first step in creating a chamber system in rhesus macaques. The thin baseplate is enclosed subcutaneously to promote healing and osseointegration without infection risks.
Treatment of D. melanogaster with small molecules in fly food
By savannah onThis protocol describes the preparation of fly food with small molecules to treat flies during their aging. It can be adapted for any treatments. Details for small molecules used can be found in the "Materials" section.
Behavior monitoring of D. melanogaster using ethoscopes
By savannah onThis protocol describes the practical steps to perform behavioral monitoring using ethoscopes. The cleaning and preparation of glass tubes as well as loading the flies into ethoscopes is outlined.
Seizures – like behavior assay for D. melanogaster
By savannah onThis protocol allows to assess the susceptibility of flies to seizure-like behavior based on mechanically hyper-stimulating sensory inputs and assessing seizure-like activity.
Genetic interaction analysis using electroretinograms (ERGs) in D. melanogaster
By savannah onThis protocols describes the recording of electroretinograms (ERGs) and calculation of genetic interactions of fly mutants for a transheterozygous screen.
Immunofluorescent staining of dopaminergic neurons in brains of D. melanogaster
By savannah onThis protocol is used to visualize dopaminergic neurons in Drosophila brains, but can be adapted for other targets as well.
Startle induced negative geotaxis (SING)
By savannah onThis protocol describes how to perform the Startle induced negative geotaxis (SING) assay. SING defects in Parkinsonism fly models have previously been shown to be caused by dopaminergic impairments.
Images of mouse VTA and SNc sections labeled for neurotransmitter phenotype markers via RNAscope
By savannah on48 coronal sections from three male and three female C57Bl/6J mice were labeled for mRNAs encoding the canonical vesicular transporters for dopamine (VMAT2), GABA (VGAT), and glutamate (VGLUT2). Imaged via confocal microscopy.
Primary data for article, “Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input” (DOI: 10.1016/j.celrep.2024.114483)
By savannah onPrimary data for the published manuscript, "Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input" (Ryan et al. Cell Reports 43:8, 114483, 2024.).
Masson-fontana staining for melanin detection
By Emma Sherrell onMasson-fontana staining for melanin detection.
