Adora2a-T2A-FlpO Mouse Line
By onAdora2a-Cre line generated by Cyagen for the Costa lab crossed with a FlpO line.
Drd1-T2A-FlpO Mouse Line
By onDopamine D1 receptor FlpO mouse line generated by the Costa lab at Cyagen using CRISPR/Cas-mediated genome engineering.
Adora2a-T2A-Cre Mouse Line
By onAdora2a-Cre line generated by Cyagen using CRISPR/Cas-mediated genome engineering for the Costa lab.
Ef1a-DIO-PSAML-141F,Y115F-GlyR-2A-FusionRed plasmid
By onPharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
RNA Seq dataset for: α-Synuclein pathology disrupts mitochondrial function in dopaminergic and cholinergic neurons at-risk in Parkinson’s disease
By onActively transcribed messenger ribonucleic acids (mRNAs) were harvested from SNc dopaminergic neurons in aSYN monomer or PFF-injected mice 12 weeks post injection and then subjected to RNASeq analysis.
pAAV-Con/Fon-N2cG
By onThis plasmid was generated by Columbia University Zuckerman Institute Molecular Tools Core Facility (RRID:SCR_026201) for our study and injected to label presynaptic inputs to SNr neurons.
pAAVDJ-Esyn-FlpX-TVA950-EYFP-WPRE
By onThis plasmid can be used to label presynaptic inputs to SNr and SNr-IC neurons.
Behavioral tasks-Falasconi & Kanodia et al 2025
By onProtocol for freely moving pellet or water reaching, lever pressing and open field behavioral assays from Falasconi & Kanodia et al 2025.
Datasets and Key Resources Table used in Bouabid, S. et al. (2025) “Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations””
By onThis repository contains a 1) Key Resources Table with details on key resources and the persistent identifiers for protocols and code used and generated in this study; 2) Source Data Folder, and 3) Supplementary Data Folder.
Fabrication and calibration of multi-fiber arrays to monitor dopamine release in the mouse striatum
By onA new method allows monitoring dopamine release in mice with high spatial and temporal resolution in multiple locations in the striatum simultaneously using a fluorescent sensor. The protocol involves array fabrication and pre-implant mapping.
Micro-CT scanning for post-implant localization of multi-fiber arrays in mouse striatum
By onNew micro-fiber array allows chronic measurement and optogenetic manipulation at 100+ locations in mice, for studying cell-type and neurotransmitter-specific signals in 3-D volumes. Protocol involves micro-CT scanning and fiber localization.
Confocal microscopy to assess TelC and iGluSnFR expression in fixed mouse brain slices
By onProtocol outlines tissue processing after glutamate recordings with iGluSnFR sensor & behavior experiments post TelC viral injection in dorsal medial striatum.
Custom Phyton scripts related to Bouabid, S. et al (2025) “Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations””
By onPhyton scripts written by Safa Bouabid and adapted from Han Lab for reading 2D treadmill ball velocity with Raspberry Pi.
Custom MATLAB scripts related to Bouabid, S. et al. (2025) “Distinct spatially organized striatum-wide acetylcholine dynamics for the learning and extinction of Pavlovian associations”
By onMATLAB scripts written by Safa Bouabid to analyse ACh release dynamics, DA release, glutamate release onto cholinergic interneurons, and behavioural changes in mouse striatum during learning and extinction of Pavlovian associations.
Multi-fiber photometry in head-fixed mice performing a dual-cue delay Pavlovian association
By onProtocol describes dual-cue Pavlovian association & multi-fiber photometry in head-fixed mice. Mice trained to link cues with rewards & undergo extinction phase with reduced rewards.
Stereotaxic injections of viral vectors and chronic optical fiber implants in mouse brains
By onProtocol details surgical steps for viral injections and optical fiber implantation in mouse striatum to monitor neurotransmitter dynamics during learning and extinction, using genetic sensors and tetanus toxin to suppress acetylcholine release.
A molecular atlas of cell-type specific signatures in the parkinsonian striatum
By onProgressive dopamine loss in PD affects striatum cells differently. Transcriptomic analysis in mouse and human models reveals changes in neuronal and glial populations, highlighting resilient and vulnerable cell types for potential new treatments.
LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
By onPD LRRK2 influences the effects of haloperidol, a common antipsychotic, on striatal indirect pathway neurons. Inhibiting LRRK2 kinase activity reduces haloperidol’s motor side effects, suggesting a way to ease antipsychotic side effects.
Mass spectrometry proteomics dataset
By onThe mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD053892
LRRK2 kinase inhibition restores haloperidol effects on rpS6 signaling in iSPNs
By onIF images in LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
