DAB staining of fixed mouse brain tissue sections
By Emma Sherrell onThis protocol describes steps for immunohistochemical 1 3,3’-Diaminobenzidine (DAB) staining of free floating fixed mouse brain tissue sections.
Optical density analysis for DAB staining of mouse brain sections
By Emma Sherrell onThis protocol describes steps for optical density analysis of immunohistochemical 3,3′-Diaminobenzidine (DAB) staining of mouse brain sections using ImageJ.
Analytical tool for spatial gene expression with 10x Visium Platform
By Emma Sherrell onAn analytical tool to be used with the 10X Visium Platform to compare spatial gene expression values.
Data for published paper, “Chronic Hyperactivation of Midbrain Dopamine Neurons Causes Preferential Dopamine Neuron Degeneration”
By Emma Sherrell onRaw data for publication titled, "Chronic hyperactivation of midbrain dopamine neurons causes preferential dopamine neuron degeneration."
Hematoxylin and Eosin Stain – FOR PARAFFIN-EMBEDDED TISSUE SECTIONS
By Emma Sherrell onThis protocol details Hematoxylin and Eosin Stain for paraffin-embedded tissue sections.
Bioluminescence imaging
By Emma Sherrell onThis protocol describes a bioluminescence stepwise imaging protocol in mice with AAV-luciferase expression in the vagal afferents using the Ivis Lumina series III imaging system.
Food intake behavior protocol
By Emma Sherrell onThis protocol describes the behavioral test to measure food intake in mice.
Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma
By Emma Sherrell onThis protocol details the Mitochondrial Antigen Presentation (MitAP) to 2CZ CD8+ T cell hybridoma.
Peripheral blood immune cells from individuals with Parkinson’s disease or inflammatory bowel disease share deficits in iron storage and transport that are modulated by non-steroidal anti-inflammatory drugs
By Emma Sherrell onParkinson's disease (PD) involves dysregulated neuroimmune crosstalk and gut-brain axis inflammation. Gastrointestinal dysfunction is a common early symptom. Iron dysregulation and immune cell dysfunction may play a role, with shared features in PD an IBD.
Behavioral screening defines three molecular Parkinsonism subgroups in Drosophila
By Emma Sherrell onThe authors created a new collection of 24 genetically well-controlled Drosophila models of familial forms of Parkinsonism. Using unbiased behavioral screening and machine learning, they identified three clusters of mutants that converge.
R scripts for the statistical analysis and plotting in the paper GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes
By Emma Sherrell onR scripts for the statistical analysis and plotting in the paper GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes.
Dataset: Parkinson’s Families Project: A UK-wide study of early onset and familial Parkinson’s disease
By Emma Sherrell onParkinson’s Families Project aims to identify genetic variations linked to familial and early-onset Parkinson's disease. SNP array genotyping, MLPA, and WGS were performed to study pathogenic mutations and their association with clinical factors.
Preparation of qPCR standards for AAV titering
By Emma Sherrell onqPCR standards for AAV titering- document annex to protocol, "AAV Purification Protocol with Iodixanol gradient."
Human brain sequential extraction (Tau)
By Emma Sherrell onThis protocol details human brain sequential extraction for pathological tau. This protocol is an adaptation of the work of several labs.
Directionality_GMX
By Emma Sherrell onThis script calculates a directionality index to assess the randomness of cell movement.
HA_Skeleton_Analysis
By Emma Sherrell onThis script assesses the complexity of extracellular matrix network through a skeleton analysis.
Characterizing enteric neurons in dopamine transporter (DAT)-Cre reporter mice reveals dopaminergic subtypes with dual-transmitter content
By Emma Sherrell onUsing a reporter mouse line expressing tdTomato under control of DAT promoter in the gut and single cell sequencing, the authors uncovered a novel population of DA neurons unique to the ENS which was ChAT/DAT-tdTomato-immunoreactive and expressed Grp, Calcb.
Raw tabular data of confocal microscopic images for “Characterizing enteric neurons in dopamine transporter (DAT)-Cre reporter mice reveals dopaminergic subtypes with dual-transmitter content”
By Emma Sherrell onUsing immunofluorescence, the authors uncovered a subpopulation of tdTomato-immunoreactive neurons in the ENS exhibit dual-transmitter content (DAT/ChAT, DAT,nNOS).
Isolation of brain infiltrating lymphocytes
By Emma Sherrell onThis protocol details the isolation of brain infiltrating lymphocytes after adoptive transfer of mitochondrial antigen- specific T cells. Splenic lymphocytes are screened to verify the presence/phenotype of CD8 in the periphery.
Pixel-dependent offset, gain, variance, and readnoise maps for CS505MUP polarization camera
By savannah onFour tif files are the pixel-dependent camera offset (in ADU counts), gain (in ADU counts/photoelectron), variance (in ADU counts^2), and readout noise (in photoelectrons) for the full sensor of a polarization camera (CS505MUP, Thorlabs).
