Neural activation of the GI tract
By savannah onProtocol for neuronal activation of the enteric nervous system.
Immunophenotyping of the peripheral blood immune cells by flow cytometry
By Julia Julia Leonard onProtocol for immunophenotyping peripheral blood immune cells.
Immunophenotyping of immune cells by high dimensional flow cytometry
By Julia Julia Leonard onProtocol to assess cellular phenotypes by multiparametric flow cytometry panels containing markers to identify cell types and activation states.
Intracardiac perfusion and rat brain fixation for immunohistochemistry
By Julia Julia Leonard onProtocol for rat brain processing to perform parallel histological analysis in one hemisphere and high dimensional flow cytometry in the other.
Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment
By Julia Julia Leonard onThis protocol is a quick guide to how to prepare a solution of LPS on the saline vehicle, how to inject a rat for an intraperitoneal injection, and how to monitor the animal after injection.
Mouse brain hemisphere organotypic cultures on glass coverslips
By Julia Julia Leonard onThis protocol describes preparation of organotypic hemisphere cultures prepared from mouse brain and cultured as rollerdrum cultures on glass coverslips.
Neuromelanin processing and image acquisition
By Julia Julia Leonard onProtocol for preparing post-mortem tissue for intracellular NM quantification.
TruAI neuromelanin quantification
By Julia Julia Leonard onQuantification of area and optical density of intracellular neuromelanin with TruAI.
Preparing 10X genomics CytAssist FFPE sections
By Julia Julia Leonard onDesigning and properly mounting FFPE sections on large-window CytAssist slides is important for downstream analysis and data quality. This protocol describes the key steps for this preparation.
Immunofluorescence on human brain FFPE sections to identify neuromelanin in A6, A9, and A10
By Julia Julia Leonard onThis is the protocol to scan brain neuromelanin with reduced impact of the chemicals on their original color and intensity. The immunofluorescence staining protocol is designed to identify human A6, A9, and A10 cell clusters and alpha-synuclein pathology.
Acute extracellular multi-unit recordings and optotagging
By Julia Julia Leonard onThis protocol details acute extracellular multi-unit recordings and optotagging in mice.
Neuromelanin quantification in stained brain slices of the Locus coeruleus
By Julia Julia Leonard onThis protocol describes an automatizable pipeline for cell counting based on fluorescent stainings with the help of CellPose. Additionally, it describes the entire generation process of plots that show the intensity of neuromelanin per cell based on bright-field images.
Juxtacellular Recordings in Ventral tegmental area and Locus coeruleus
By Julia Julia Leonard onThis protocol describes juxtacellular recordings in the VTA and LC of adult mice, involving anesthesia, precise brain targeting, and neuron labeling. Using specialized electrodes and a dual-channel amplifier, neuronal activity is recorded and modulated with current injections. Following recordings, brains are perfused, fixed, and sectioned for detailed morphological analysis.
Systemic inflammation triggers long-lasting neuroinflammation and accelerates neurodegeneration in a rat model of Parkinson’s disease overexpressing human alpha-synuclein
By Julia Julia Leonard onIncreasing efforts have been made to elucidate how genetic and environmental factors interact in Parkinson's disease (PD). In the present study, the authors assessed the development of PD-like symptoms on a PD rat model overexpressing human α-synuclein at a presymptomatic age, exposed to a pro-inflammatory insult by injection of lipopolysaccharide.
Sucrose preference test
By Julia Julia Leonard onThe sucrose preference test assesses the animal’s interest in a sweet-tasting sucrose solution relative to unsweetened water.
Immunohistochemistry free-floating rat brain cryosections
By Julia Julia Leonard onProtocol for immunohistochemistry on rat brain cryosections.
Immunofluorescence free-floating rat brain cryosections
By Julia Julia Leonard onProtocol for immunofluorescence on rat brain cryosections.
Optical densitometry of tyrosine hydroxylase fibers
By Julia Julia Leonard onProtocol for quantifying TH+ fibers in rat striatum and substantia nigra pars reticulata (SNpr).
Constant potential amperometry in vitro
By Julia Julia Leonard onProtocol for constant potential amperometry recordings in vitro of evoked dopamine release in the rat striatum.