Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
By onCryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
Lipid dynamics at membrane contact sites
By onLipid building blocks in eukaryotes are mainly produced in the endoplasmic reticulum and moved to other organelles via vesicles or proteins at organelle contact sites. We review what is known.
Quantification of Axonal Projections from Whole-Brain Slide Images
By onQuantification of Axonal Projections from Whole-Brain Slide Images provides a standardized method for processing and analyzing immunofluorescence images of mouse brain sections to quantify axonal projections.
IBA1 Immunochemistry on human paraffin sections
By onIba1-specific Immunochemistry protocol on paraffin-embedded human brain sections
Olympus Slideview VS200″ slide scanning
By onBrief guide on the main steps to follow in order to scann a sample with the "Olympus Slideview VS200 slide scanner. Although examples for certain parameters are presented, each user should choose them based on the characteristics of their samples.
Cytokine Array
By onThe EMD MILLIPLEX‱ MAP Human Cytokine/Chemokine/Growth Factor Pane A – Immunology Multiplex Assay (Merck Millipore) is employed to simultaneously analyse TNF-α, IL-1β, IL 6, and INFγ with Bead-Based Multiplex Assay using the Luminex‱ technology.
Gene expression analysis
By onTo evaluate whether human microglia cells (hMG) can be activated by neuromelanin (NM), hMG were cultured with and without the presence of NM for 24 h and then harvested to measure gene expression of selected pro-inflammatory and neurotoxic factors.
Immunocytochemistry (ICC) detection of key microglia markers in iPSC derived human microglia cells
By onImmunocytochemistry (ICC) detection of key microglia markers in iPSCderived human microglia cells (hMG)
Intracellular ROS measurement in iPSC-derived microglia
By onIntracellular ROS measurement in iPSC-derived microglia
Isolation of pure neuromelanin extracts from cultured TR5TY6 neuroblastoma cells
By onIsolation of pure neuromelanin extracts from cultured TR5TY6 neuroblastoma cells
LRRK2-mutant microglia and neuromelanin synergize to drive dopaminergic neurodegeneration in an iPSC-based Parkinson’s disease model
By onPD is a neurodegenerative disorder characterized by the loss of neuromelanin (NM)-containing dopamine neurons. This work highlights NM-activated microglia’s role in PD progression, and provides a model for testing therapeutic targets.
Adeno-associated viral vectors for modeling Parkinson’s disease in non-human primates
By onThe lack of adequate animal models of PD represents the main barrier to preclinical therapies to succeed in clinical trials. However, by novel generations of viral vectors coding for different forms of a-syn species and related genes.
