Microscopy-based pUb-coverage measurements of mitochondria in iNeurons
By onAssociated with preprint:
A prebiotic diet modulates microglia response and motor deficits in α-synuclein overexpressing mice
By onSingle-cell RNA-seq analysis of microglia from the substantia nigra and striatum uncovers increased pro-inflammatory signaling and reduced homeostatic responses in ASO mice compared to wild-type counterparts on standard diets. However, prebiotic feeding reverses pathogenic microglial states in ASO mice and promotes expansion of protective disease-associated macrophage (DAM) subsets of microglia. Notably, depletion of microglia using a CSF1R inhibitor eliminates the beneficial effects of prebiotics by restoring motor deficits to ASO mice despite feeding a prebiotic diet. These studies uncover a novel microglia-dependent interaction between diet and motor symptoms in mice, findings that may have implications for neuroinflammation and PD.
CRISPR/Cas9 generation of knock-out iPSCs
By onThis protocol describes RNP-based CRISPR/Cas9 gene-editing to generate knock-out iPSCs.
Brain Slice Preparation for electrophysiology recording
By onThis protocol describes brain slice preparation for electrophysiology recording.
LiD genetic determinants study under CPH regression models
By onCode to perform the study of LiD genetic determinants under CPH regression models and functional annotation analyses.
Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
By onThe results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.
Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”
By onThe files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.
Mouse Behavior – Open Field and T-Maze
By onThis protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
Scientific Perspectives: Structural Biology of LRRK2 and its Interaction with Microtubules
By onMutations in LRRK2 are linked to Parkinson's disease. LRRK2 regulates membrane trafficking and interacts with microtubules. Recent studies have revealed its cytosolic and microtubule-bound forms using cryo-EM and cryo-ET techniques.
HEK293 TMEM115-3xHA GolgiTAG (CVCL_C8A6)
By onCell line generated from HEK293 cells (Cat# CRL-1573, RRID:CVCL_0045) virally transfected with a construct expressing TMEM115-3xHA (GolgiTAG)
Role of autophagy pathway in Parkinson’s disease and related Genetic Neurological disorders
By onThe authors provide a comprehensive overview of the general importance of autophagy in Parkinson’s disease (PD) and related disorders of the central nervous system (CNS).
Scalable, flexible carbon fiber electrode thread arrays for three-dimensional probing of neurochemical activity in deep brain structures of rodents
By onThe authors' CFET array has the potential to unlock a wide range of applications, from uncovering the role of neuromodulators in synaptic plasticity, to addressing safety barriers in clinical translation toward diagnostic and adaptive treatment in PD
Mechanisms Controlling Selective Elimination of Damaged Lysosomes
By onLysophagy, triggered by membrane rupture, involves galectins binding to lysosomal contents to promote autophagic recycling. Damaged lysosomes are ubiquitylated, leading to autophagosome formation for degradation in healthy lysosomes.
iPSC differentiation into Macrophages
By onThis protocol describes iPSC differentiation into macrophages.
