Plasmid Construction and Gibson cloning
By onThis protocol describes plasmid construction using Gibson cloning.
Histology Albarran et al 2025
By onHistology protocol for Albarran et al 2025 that was adapted from Fushiki et al 2024 , to quantify Th+ cells.
Generation of knock-in Cre and FlpO mouse lines for basal ganglia and dopamine neuron investigation (SOURCE DATA)
By onThe dataset contained in this repository are the processed data and information used in all the quantifications, tables, and figures associated with the project by Albarran et al 2025 elife.
Albarran et al Histology dataset
By onDataset from a whole-brain imaging experiment showing recombinase expression in different brain regions using knock-in lines (D1-Cre, A2a-Cre, D1-FlpO, A2a-FlpO, DAT-FlpO) crossed with fluorescent reporter lines.
A Multimodal Atlas Reveals the Anatomical Distribution of Medium Spiny Neuron Subtypes and a Novel RGS6+ Population in the Primate Striatum
By onWe combined single-nucleus multi-omic sequencing and high-plex spatial transcriptomics to build a comprehensive atlas of medium spiny neurons (MSNs) across the entire macaque striatum, revealing new MSN subtypes and their association with diseases.
Iron mishandling in the brain and periphery in Parkinson’s disease
By onThe prodromal phase of Parkinson’s disease is complex. Gastrointestinal dysfunction and iron dysregulation may drive neurodegenerative risk. Identifying catalysts in the gut is crucial for developing disease-modifying therapies.
Cryo-electron tomography of thinned synapses
By onProtocol for cryo-ET of thinned synapses in rat neuron cultures is detailed. Cryo-FIB milling creates thin samples for high-resolution imaging. Synapse targeting with and without cryo-FLM correlation is explained.
Single particle analysis of α-Synuclein fibrils
By onAggregates of α-Synuclein are linked to Parkinson’s, multiple system atrophy, and Lewy body dementia. This protocol outlines near-atomic resolution structures of these amyloid fibrils using cryo-EM single particle analysis.
Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography
By onSandwich hippocampal neuron cultures have low cell density and few glial cells, making them ideal for targeting neurons for cryo-electron tomography.
Single particle analysis datasets of in vitro grown aSyn filaments
By onDataset includes cryo-EM micrographs of a-Synuclein fibrils with WT and mutant forms (G14R, K58N) found in patients.
Tomogram of aggregate in AgDD-sfGFP-expressing HEK293 cell 10 min post aggregation induction
By onAgDD protein aggregation can be controlled for cellular studies. A cryo-electron tomogram shows an AgDD aggregate in a HEK293 cell 10 min post-aggregation induction.
