Assembloid model to study loop circuits of the human nervous system
By Emma Sherrell onHuman stem cells are used to create in vitro cortico-striatal-thalamic-cortical circuits, mimicking neural networks involved in neuropsychiatric disorders. This system allows the study of normal development and diseases like autism and Tourette syndrome.
T cell purification and activation
By Emma Sherrell onThis protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen.
Global TMTpro proteomics on iNeuron and HeLa FBXO7 cells studying mitophagy
By Emma Sherrell onDataset includes proteomic analysis of iNeurons at day 0, 4, 12, with and without FBXO7, and HeLa cells under fed and mitophagy conditions, with and without FBXO7.
Maintenance & differentiation: Embryonic mouse hippocampal cells (CLU198)
By Emma Sherrell onThis protocol outlines the maintenance, differentiation, and harvesting of the CLU198 embryonic mouse hippocampal cell line.
MSD V-PLEX Proinflammatory panel
By Emma Sherrell onThe V-PLEX panel offers analytically validated multiplex assay kits. Developed under rigorous design control, V-PLEX kits provide accurate and reproducible results with consistency from lot to lot. The V-PLEX Proinflammatory Panel 1 (human) Kit includes ten biomarkers that are associated with the inflammatory response and immune system regulation.
Bulk tissue RNA-sequencing – hiPSC-derived astrocytes, neurons, and co-cultures
By savannah onBulk tissue RNA-sequencing of hiPSC-derived astrocytes, neurons, and co-cultures using the Illumina TruSeq Stranded mRNA Library Prep kit.
Electrophysiology with iPSC-derived neurons
By savannah onThis protocol describes the method to perform patch-clamp recordings of iPSC-derived neurons.
Peripheral immune neurodegeneration
By Emma Sherrell onThe project investigates if immune cells impact neurodegenerative diseases differently based on ancestry. It examines disease gene expression and genetic links in African, East Asian, and European populations.
Aggregation of human recombinant alpha-synuclein
By savannah onThis protocol describes a method of generating aggregation of human recombinant alpha-synuclein.
Validating responsiveness of AAV-DIO-hM3Dq-DREADD
By Emma Sherrell onProtocol for validating behavioral response to IP injection of clozapine-n-oxide in DAT-IRES-Cre mice injected with AAV-DIO-hM3Dq-DREADD in midbrain.
Ex vivo electrophysiology
By Emma Sherrell onThis protocol describes steps for ex vivo electrophysiology in mouse brain slices. This protocol also includes instructions for clozapine N-oxide (CNO) testing in DREADD-expressing neurons.
Datasets related to “Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits”
By savannah onDatasets related to "Loss of the lysosomal lipid flippase ATP10B leads to progressive dopaminergic neurodegeneration and Parkinsonian motor deficits."
Photometry acquisition in freely moving mice
By Emma Sherrell onThis protocol describes the steps for acquiring photometry data for freely moving mice.
ASO transfection of iPSC-derived cells
By savannah onA protocol for transfecting iPSC-derived midbrain dopaminergic neurons with antisense oligonucleotides (ASOs).
Immunofluorescence staining of fixed mouse brain tissue sections
By Emma Sherrell onThis protocol describes steps for immunofluorescent staining of free floating fixed mouse brain tissue sections.
