LRRK2 alters the composition and organization of active zone release sites in dopaminergic striatal synaptosomes
By onConfocal images and analysis overview
TH fibers and dopamine vesicle clustering alterations
By onTh fibers and dopamine vesicle clustering alterations in Lrrk2WT and Lrrk2G2019S mice
LRRK2 is expressed in dopamine neurons and is enriched in the vulnerable subpopulation
By onConfocal images from Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
LRRK2 disrupts the number and composition of active zone release sites in vulnerable dopamine axons
By onStriatal brain sections were stained with antibodies against bassoon to analyze the number and organization of active zone sites in Aldh1a1+ (EYFP+) or Aldh1a1- (mCherry+) axons from the same mouse
Immunofluorescence and confocal imaging in brain sections and image analysis
By onImmunofluorescence and confocal imaging in brain sections and image analysis for Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Axonal proteome analysis and Western Blot via APEX2 labeling
By onAxonal proteome analysis and western blot via APEX2 labeling related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
SIM imaging and analysis for active zone release sites in vulnerable dopamine axons
By onStereotactic injections and viruses described and SIM imaging and analysis
Liquid Chromatography-Mass Spectrometry (LCMS) with striatal synaptosomes
By onLiquid Chromatography-Mass Spectrometry (LC-MS) with striatal synaptosomes assay related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons preprint
Western blot and Phostag gels
By onWestern blot and Phostag gels protocol related to Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Proteomics for STING KO and STINGRRAA RAW 264.7 macrophages
By onUsing immunopeptidomics analysis we showed that STING regulates the repertoire of peptides displayed at the cell surface of macrophages during inflammation, highlighting a potential role in immunosurveillance and potential targets for therapy
Striatal synaptosome preparation for imaging
By onStriatal synaptosome imaging and analysis protocols in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Experiments in HEK-293 cells
By onProtocols related to experiments in HEK-293 cells in Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons preprint
Custom scripts related to Graziano, M. et al. (2025) “A molecular atlas of cell-type specific signatures in the parkinsonian striatum”
By onScripts by Graziano and Masarapu aid in analyzing single-nucleus RNA sequencing data from Parkinson's Disease striatum, mapping vulnerabilities, and inferring cell trajectories. Giacomello Lab scripts are available on GitHub.
Code used in “Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitis”
By onUsing scRNAseq and flow cytometry, we demonstrated that the Lrrk2-G2019S mutation is associated with an increased neutrophil presence in the colonic lamina propria, Th17 skewing, upregulated Il17a, and greater colonic pathology during infection.
Transcriptomic analysis of colonic lamina propria cells in LRRK2 G2019S mice compared to wild type following intestinal microbial infection
By onUsing scRNAseq and flow cytometry, we demonstrated that the Lrrk2 G2019S mutation is associated with an increased neutrophil presence in the colonic lamina propria during infection and a Th17 skewing, upregulated Il17a, and greater colonic pathology
Re-activation of neurogenic 2 niches in aging brain
By onWe developed a multimodal MERFISH platform to map cell fate in the brain, revealing quiescent neural stem cells in aged mice and humans. PTBP1 suppression reactivates them, driving neurogenesis and neuron integration with therapeutic potential.
Rapid LRRK2 Activation Induced by α-synuclein Preformed Fibrils Triggers Rab5 Phosphorylation and Dysregulates Endolysosomal Function, Gene Expression and Synaptic Activity in Neurons
By onα-Synuclein fibrils activate LRRK2 on early endosomes, disrupting Rab5 function and endolysosomal homeostasis in neurons, triggering chromatin changes and transcriptional reprogramming.
Single-nucleus multiomic profiling of the aging mouse substantia nigra reveals conserved gene alterations linked to Parkinson’s disease
By onSingle-nucleus analysis of mouse substantia nigra reveals cell-type-specific aging changes, highlighting PD-linked genes. Aging alters protein folding, myelination, and stress-response pathways, offering insights into PD risk.
Activation of transposable elements is linked to a region- and cell-type-specific interferon response in Parkinson’s disease
By onStudy explores transposable elements role in Parkinson's disease neuroinflammation. TE activation in specific brain regions correlates with innate immune responses, indicating potential involvement in chronic neuroinflammation and PD progression.
Tailored workflows for the analysis of transposable elements (Garza et al, 2025)
By onGarza et al. (2025) created customized workflows for transposable element analysis across various data types. Workflows are in Snakemake, Jupyter notebooks, R markdowns, and scripts. Details are in the GitHub README.
