GRIN lens implant (1 mm) Protocol
By onThis is the standard 1 mm GRIN lens implant protocol for Rodrigues-Vaz and Athalye et al, 2025.
EMG recordings-Rodrigues-Vaz and Athalye et al, 2025
By onThis is the standard protocol for recordings of bulk EMG used for Rodrigues-Vaz and Athalye et al, 2025.
Perfusion and Histology Rodrigues-Vaz and Athalye et al, 2025
By onThis is the standard protocol for perfusion and histology of brain slices for Rodrigues-Vaz and Athalye et al, 2025.
Isometric forelimb task behavioral training
By onThis task is a self-paced task in which mice are reinforced for using their forelimb to apply force to an immobile joystick exceeding a threshold of duration and force.
Holographic calibration
By onThis is the standard protocol for 2-photon holographic calibration with In vivo Ultima Bruker microscope through GRIN lens used for Rodrigues-Vaz and Athalye et al, 2025.
Imaging using 2-photon microscope through GRIN lens
By onThis is the standard protocol for 2-photon imaging with Bruker microscope through GRIN lens used for Rodrigues-Vaz and Athalye et al, 2025.
Matters arising: Forced Symmetry Artifacts in a Prohibitin Complex Structure
By onLange et al. proposed a model for mitochondrial prohibitin complex with unequal subunit stoichiometry. Data suggests an asymmetric structure, questioning the validity of the proposed model. Care is needed in symmetry imposition in cryo-ET.
Immunoprecipitation of NAP1-GFP
By onThis protocol describes the procedure to perform immunoprecipitation of NAP1-GFP from HAP1 cells.
