Brain infiltrating leukocytes (BILs) extraction
By Emma Sherrell onThis protocol details the extraction of brain infiltrating leukocytes (BILs).
Gut microbiome-mediated regulation of neuroinflammation
By Emma Sherrell onGut microbiome influences neuroinflammatory disease through pathways, including metabolite production, immune cell trafficking, and vagus nerve. Research on the gut-brain axis has promising implications for understanding and treating neuroinflammation.
Computational analysis for “Comparative Analysis of AAV Serotypes for the Transduction of Olfactory Sensory Neurons” by Belfort and Jia et al. 2024
By Emma Sherrell onCode for snRNAseq dataset analysis of AAV-infected mouse olfactory epithelium. This code accompanies the manuscript: Comparative Analysis of AAV Serotypes for the Transduction of Olfactory Sensory Neurons by Belfort, Jia et al. 2024.
Single nucleus transcriptomic dataset of AAV-infected mouse OE
By Emma Sherrell onSingle nucleus sequencing dataset from mouse murine olfactory epithelium exposed to 4 AAV serotypes via nasal lavage. The goal was to identify the best candidate for transduction of olfactory sensory neurons. Code is included with the dataset.
Astrocyte generation
By Emma Sherrell onA protocol for generating astrocytes from cortical neural progenitor cells using a commercial astrocyte media (AM).
Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons
By Emma Sherrell onThis study compared the efficacy and selectivity of 11 different AAV serotypes for the efficient and selective transduction of murine olfactory sensory neurons in vivo. The findings inform research approaches and gene therapy-based initiatives.
Microscopy dataset for: Comparative Analysis of AAV Serotypes for Transduction of Olfactory Sensory Neurons
By Emma Sherrell onImaging dataset for the manuscript, "Comparative Analysis of AAV Serotypes for Transduction of Olfactory Sensory Neurons" by Belfort, Jia et al., 2024.
Endo-IP and Lyso-IP toolkit for endolysosomal profiling of human induced neurons
By Emma Sherrell onThe endolysosomal system regulates plasma membrane protein degradation and recycling, crucial for cell surface protein abundance. A toolkit was developed to study endosomes and lysosomes in neurons, aiding in understanding neurodegenerative diseases.
Making Ca2+ – selective microelectrodes
By Emma Sherrell onProtocol for making Ca2+ sensitive electrodes to measure extracellular Ca2+ concentrations at high spatiotemporal resolution in acute ex vivo brain slices.
Intracranial injections of viral vectors in mouse striatum
By Emma Sherrell onProtocol for intracranial viral vector injections in dorsal or ventral striatum in mice is outlined. Steps are generalizable but require optimization for injection coordinates and virus concentration.
Custom Phyton scripts related to Stedehouder & Roberts (2024) “Rapid modulation of striatal cholinergic interneurons and dopamine release by satellite astrocytes”
By Emma Sherrell onScripts by Jeffrey Stedehouder and Professor Kevin McGerty to analyze and plot FCV and patch-clamp experiments in ex vivo mouse brain slices and to model the distance between cell types.
Abnormal hyperactivity of specific striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering
By Emma Sherrell onL-DOPA-induced dyskinesia is a common issue in Parkinson's disease due to dopamine therapy. These findings show that ensembles of behavior-encoding D1- and D2-SPNs form new combinations of hyperactive neurons mediating specific dyskinetic movements.
Protocols for stereotaxic injections into mouse brain and ex-vivo electrophysiology
By Emma Sherrell onThe protocol outlines the injection of α-Synuclin PFF and monomer into the mouse brain, followed by the preparation of acute slices from mice for whole-cell patch clamp recordings.
α-Synuclein seeding activity and progression in sporadic and genetic forms of Parkinson’s disease in the Parkinson’s Progression Markers Initiative cohort
By Emma Sherrell onBaseline α-syn seeding activity and seed concentration were not statistically significantly associated with PD progression in the PPMI cohort.
Designing sgRNA oligos and inserting guides into the GEARBOCS vector
By Emma Sherrell onProtocol for designing sgRNAs for genes using GEARBOCS vector backbone enabling astrocyte-specific genetic manipulation in CRISPR/Cas9 mice for in vivo assays.
Behavioral analyses in mice
By Emma Sherrell onThis collection of protocols describes different behavioral assays performed in mice, including grip strength test, pole test, rotarod, open field, and nose poking learning task.
DAB immunohistochemistry (IHC) staining for stereological analysis
By Emma Sherrell onThis protocol details the DAB immunohistochemistry staining for stereological analysis of 40 µM slices cut with cryostat and stored in antifreeze.
Immunohistochemistry of free floating slices
By Emma Sherrell onThis protocol details the immunohistochemistry of free-floating slices.
Perfusion/fixation for cryostat slicing for HHC
By Emma Sherrell onThis protocol details the Perfusion and Fixation for cryostat slicing for HHC.
DIO-SPOTlight transgenic mouse to functionally monitor protein synthesis regulated by the integrated stress response
By Emma Sherrell onA new SPOTlight reporter allows visualization of the integrated stress response (ISR) activity in specific cell types in transgenic mice, aiding in studying health and disease implications.
