Glucosylceramide and glucosylsphingosine analysis V.2
By Emma Sherrell onThis protocol was used to analysis glucosylceramide and glucosylsphingosine levels in mouse brains and liver. It was also described in a previous publication (Mol Cell Neurosci. 2020 Jan:102:103451, doi: 10.1016).
Mouse tissue collection
By Emma Sherrell onThis protocol is about collecting mouse tissues for immunohistochemistry or biochemistry experiments. While it is mainly used for brain tissues, it also can be used for other visceral tissues.
Immunohistochemistry data processing
By Emma Sherrell onThis protocol has been used for high throughput quantification of immunohistochemistry data of mouse brain sections.
Micro-PET CT procedures for brain imaging of rats
By Emma Sherrell onMicro-PET CT procedures for brain imaging of rats.
Western blot for tissue extract
By Emma Sherrell onProtocol for the detection of proteins by Western blot from tissue extract.
Endogenous LRRK2 and PINK1 function in a convergent neuroprotective ciliogenesis pathway in the brain
By Emma Sherrell onPreprint: Mutations in LRRK2 and PINK1 are associated with familial Parkinson's disease (PD). The present study indicates that LRRK2 activation or loss of PINK1 function along parallel pathways impairs ciliogenesis, suggesting a convergent mechanism toward PD.
Protocol for performing PINK1 siRNA knockdown in mouse embryonic fibroblasts (MEFs)
By Emma Sherrell onThis protocol details the siRNA knockdown in mouse embryonic fibroblasts (MEFs) for PINK1, but applicable for any other target.
RNA extraction and PCR protocol
By Emma Sherrell onThis protocol details the extraction of RNA and PCR analysis.
Molecular and spatial transcriptomic classification of midbrain dopamine neurons and their alterations in a LRRK2G2019S model of Parkinson’s disease
By Emma Sherrell onPreprint: The authors developed an optimized pipeline for single-nuclear RNA sequencing (snRNA-seq) and generated a high-resolution hierarchically organized map revealing 20 molecularly distinct DA neuron subtypes. The data revealed heterogeneity even within neuroanatomical sub-structures. Finally, in a preclinical LRRK2G2019S knock-in mouse model of PD, subtype organization and proportions are preserved.
Synapse function and gene enrichment analysis.
By Emma Sherrell onOnline tool for the exploration of snRNA sequencing dataset of dopamine neurons.
Figure 2. Rubicon and Pacer RH domain have inverted pRAB7A binding specificity [FINAL]
By savannah onFigure 2. Rubicon and Pacer RH domain have inverted pRAB7A binding specificity.
Adult-onset deletion of ATP13A2 in mice induces progressive nigrostriatal pathway dopaminergic degeneration and lysosomal abnormalities
By Emma Sherrell onPreprint: The study demonstrates that the adult-onset homozygous deletion of ATP13A2 in the nigrostriatal pathway produces robust and progressive dopaminergic neurodegeneration that serves as a useful in vivo model of ATP13A2-related neurodegenerative diseases.
Passaging and seeding Mouse embryonic fibroblasts (MEFs) for experiments
By Emma Sherrell onThis protocol details the passaging and seeding mouse embryonic fibroblasts (MEFs) for experiments.
Preparation of free floating coronal mouse brain sections
By Emma Sherrell onThis protocol details the preparation of free floating coronal mouse brain sections.
Mouse stereotaxic surgery
By Emma Sherrell onThis protocol details the mouse stereotaxic injections into the mouse brain.