Cytotoxicity Assay Protocol
By Emma Sherrell onThis is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.
Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By Emma Sherrell onHolding ACSF is used for incubating brain slices before cell-attached or whole-cell electrophysiological recordings of neurons.
Preparation of Recording ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By Emma Sherrell onRecording ACSF is used for incubating brain slices during cell-attached or whole-cell electrophysiological recordings of neurons.
v2 RNAscope
By Emma Sherrell onThis is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD).
Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging
By Emma Sherrell onAβ peptides derived from the amyloid precursor protein (APP) have been implicated in the pathogenesis of Alzheimer’s disease. However, the normal function of APP is less clear. Results demonstrate a conserved role for APP in controlling age-dependent proteostasis with plausible relevance to Alzheimer’s disease.
Imaging and analysis of mouse midbrain tissue labeled by RNAscope
By Emma Sherrell onThe authors describe imaging, analysis, and cell counts of coronal sections of mouse substantia nigra and ventral tegmental area labeled with Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).
Preparation and RNAscope-labeling of fresh mouse midbrain tissue
By Emma Sherrell onThe authors describe preparing and labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for probes targeting the genes Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).
LC/MS analysis of plasma samples from PPMI
By Emma Sherrell onPlasma samples from PPMI were analyzed by liquid chromatography with mass spectrometry (LC/MS) for a variety of metabolites (including piperine) and lipids as interrelated markers of Parkinson's disease and its pathophysiology.
Modeling gene-environment interactions in Parkinson’s disease: Helicobacter pylori infection of Pink1-/- mice induces CD8 T cell-dependent motor and cognitive dysfunction
By Emma Sherrell onParkinson's disease (PD) is a neurodegenerative disorder characterized by loss of motor function. Using a mouse model, the authors demonstrate that infection with Helicobacter pylori leads to the development of motor and cognitive signs.
Purification of total RNA from cells using spin technology
By Emma Sherrell onPurification of total RNA from cells using spin technology.
Large-scale visualization of α-synuclein oligomers in Parkinson’s disease brain tissue
By Emma Sherrell onParkinson’s disease is a neurodegenerative condition characterized by large intraneuronal aggregates in the brain. It has been hypothesized that these large aggregates may form from smaller soluble protein assemblies, often termed oligomers. ASA-PD, is a new imaging method to generate large-scale α-synuclein oligomer maps in post-mortem human brain tissue.
The VEGFs/VEGFRs system in Alzheimer’s and Parkinson’s diseases: Pathophysiological roles and therapeutic implications
By Emma Sherrell onThis article focuses on the VEGFs/VEGFRs involvement in neurodegenerative diseases by reviewing the current literature on the rather complex VEGFs/VEGFRs contribution to the pathogenic mechanisms of Alzheimer’s (AD) and Parkinson’s disease (PD).
QSBB Digital Pathology Resource
By Emma Sherrell onThis digital pathology resource contains digital whole slide images from Lewy body disorders: Parkinson’s disease, Parkinson’s disease with dementia, dementia with Lewy bodies, and control cases.
Nova-ST: Nano-patterned ultra-dense platform for spatial transcriptomics
By Emma Sherrell onExisting spatial transcriptomics techniques are either limited by capture array density or are cost-prohibitive for large-scale atlasing. Nova-ST, a dense nano-patterned spatial transcriptomics technique derived from randomly barcoded Illumina sequencing flow cells enables customized, low-cost, flexible, and high-resolution spatial profiling of large tissue sections.
Source data for Mendonça et al 2024
By Emma Sherrell onSource data and code from dopamine neuron activity encode the length of upcoming contralateral movement sequences by Mendonça et al 2024.
Analysis of glycosphingolipids from animal tissues
By Emma Sherrell onInterest in the role of cellular glycosphingolipids (GSLs) in health and disease led the authors to develop a sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.
pMX-IG-ATG3 (K62D/K64D)
By Emma Sherrell onPlasmid: Expresses untagged ATG3 (K62D/K64D) in mammalian cells.
Plotting interspike intervals (ISI) and spikes from fast-scan cyclic voltammetric (FSCV) electrochemical and electrophysiological recordings
By Emma Sherrell onCode for plotting interspike intervals (ISI) and spikes from fast-scan cyclic voltammetric (FSCV) electrochemical (EChem) and electrophysiological (EPhys) recordings.
Task data for plotting interspike intervals (ISI) and spikes from fast-scan cyclic voltammetric (FSCV) electrochemical and electrophysiological recordings
By Emma Sherrell onData for plotting interspike intervals (ISI) and spikes from fast-scan cyclic voltammetric (FSCV) electrochemical (EChem) and electrophysiological (EPhys) recordings.