Imaging axonal calcium dynamics in ex vivo mouse brain slices

By Yanfeng Zhang, PhD and Stephanie Cragg, PhD
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Output Details

Description
This protocol describes how to image calcium dynamics in striatal dopaminergic axons in ex vivo mouse brain slices. We imaged calcium transients in response to single and trains (4 pulses, 100 Hz) of electrical stimulus pulses using genetically encoded calcium indicator GCaMP6f expressed in DAT-Cre:Ai95D mice.
Identifier (DOI)
10.17504/protocols.io.81wgbx9eolpk/v1
Tags
  • Calcium
  • Ex Vivo
  • Microscopy - optical
  • Mouse
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

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